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BACTERIAL HEMICELLULASE

CAS NUMBER: 9025-56-3

EC NUMBER: 232-799-9

 


Bacterial hemicellulases are a diverse group of enzymes that hydrolyze hemicelluloses — one of the most abundant groups of polysaccharide in nature. These enzymes have many biotechnological applications and their structure/function relationships are a subject of intense research. During the past year, new high-resolution structures of catalytic and non-catalytic domains of hemicellulases have been elucidated, and, together with biochemical studies, they reveal the principles of catalysis and specificity for these enzymes.
Bacterial hemicellulases are key components in the degradation of plant biomass and carbon flow in nature. Hemicellulases are responsible for the degradation of hemicelluloses, such as xylans, xyloglucans, arabinoxylans, and glucomannans, from plant biomass [58]. The variable structure and organization of hemicellulose requires the concerted action of many enzymes for its complete degradation. Many of the hemicellulases are modular proteins, and, in addition to their catalytic domains, include other functional modules. The most important modules are carbohydrate-binding modules, which facilitate the targeting of the enzymes to the insoluble polysaccharides, and dockerin modules that mediate the binding of the catalytic domains via cohesin–dockerin interactions, either to the microbial cell surface or to large enzymatic complexes, such as the cellulosome [59–61].
Bacterial hemicellulases commonly share similar activities with cellulases because of the common β-1,4-glycosidic bonds in the backbone of the hemicellulose component of plant biomass [62]. One of the main catalytic modules of hemicellulases is glycoside hydrolases (GHs) that hydrolyze glycosidic bonds. The hemicellulose substrate is a complex carbohydrate structure consisting of different easy hydrolysable polymers such as pentoses (e.g., xylose and arabinose), hexoses (e.g., mannose, glucose, and galactose), and sugar acids. Another main catalytic module of hemicellulases is carbohydrate esterase, which hydrolyzes ester linkages of acetate or ferulic acid side groups. Xylanases, α-d-glucuronidases, α-l-arabinofuranosidases, α-d-galactosidases, β-xylosidases, and β-mannanases attack glycosidic bonds, whereas acetyl or feruloyl esterases hydrolyze ester bonds of acetate or ferulic acid side groups in the plant cell wall structure [63].

Bacterial hemicellulases are produced by a wide spectrum of microbes in nature. Isolation of hemicellulase-producing microbes from nature is an important way to discover new hemicellulases with different properties or mine new genes coding hemicellulases. Generally, hemicellulases are produced by saprophytic microbes, which grow on dead and decaying materials. Hemicellulases from fungi and thermophilic bacteria are of commercial importance as they can usually resist robust conditions such as extreme temperatures and pH. Hemicellulase-producing bacteria were isolated from various niches including soil, parks, and forest. 
Bacterial hemicellulase is a gelling and thickening agent derived from cellulose. 

Bacterial hemicellulase is widely used in cosmetics, cleaning solutions, and other household products.
Bacterial hemicellulase and methyl cellulose are frequently used with hydrophobic drugs in capsule formulations, to improve the drugs' dissolution in the gastrointestinal fluids. 

Bacterial hemicellulase is also used extensively in the oil & gas industry as a drilling mud additive under the name HEC as well in industrial applications, paint & coatings, ceramics, adhesives, emulsion polymerization, inks, construction, welding rods, pencils and joint fillers.
Bacterial hemicellulase is one of the main ingredients.

Bacterial hemicellulaseis also a key ingredient in the formation of big bubbles as Bacterial hemicellulase possesses the ability to dissolve in water but also provide structural strength to the soap bubble.
Among other similar chemicals, Bacterial hemicellulase is often used as slime.

Bacterial hemicellulase is a commonly used cellulose ethers organic water-based ink thickening agent, belongs to a water-soluble non-ionic compound, with good water thickening ability, degraded by oxygen, acid and enzyme, under alkaline conditions can be crosslinked by Cu2+. 
Has thermal stability, when heated, does not appear gelation, does not occur precipitation under acidic conditions, the film-forming property is good, the aqueous solution can be made of a transparent film, can be derived from the reaction of alkali cellulose with ethylene oxide, having properties such as thickening, emulsifying, adhesive, suspension, film-forming, maintaining moisture and protectiving colloid. 

The role of thickener in the aqueous ink is thickened.
The viscosity of the ink added a thickening agent increases, can improve the physical and chemical stability of the ink; due to the increased viscosity, rheology of the ink can be controlled at the time of printing; the pigment and filler in ink is not easy to precipitate, increasing the storage stability of the water-based ink.

Thickening agent is a cellulose-based material and (or) polyvinyl alcohol substances. 
Bacterial hemicellulases may be methyl cellulose, ethyl cellulose, hydroxymethyl cellulose, carboxymethyl cellulose, Bacterial hemicellulase, Bacterial hemicellulase and hydroxypropylmethyl cellulose; polyvinyl alcohol material may be an or several species of polyethylene 400, 600, 800, 1000, 1600, 2000, 4000, 6000.

Bacterial hemicellulase is white to yellowish fibrous or powdery solid, non-toxic, tasteless and soluble in water. 
Insoluble in common organic solvents. 

Having properties such as thickening, suspending, adhesive, emulsifying, dispersing, water holding. 
Different viscosity range of solution can be prepared. Having exceptionally good salt solubility to electrolyte.

Bacterial hemicellulase is a thickener, protective colloid, binder, stabilizer, and suspending agent. 
Bacterial hemicellulaseis obtained from wood pulp Copyright 2014 Cengage Learning. 

Bacterial hemicellulase is primarily used as a thickening agent in ophthalmic and topical formulations, although it is also used as a binder and film-coating agent for tablets.
Bacterial hemicellulase is present in lubricant preparations for dry eye, contact lens care, and dry mouth.

The concentration of Bacterial hemicellulase used in a formulation is dependent upon the solvent and the molecular weight of the grade.
Bacterial hemicellulase is also widely used in cosmetics.

Bacterial hemicellulase is insoluble in most organic solvents. 
Bacterial hemicellulase is incompatible with zein and partially compatible with the following water-soluble compounds: casein; gelatin; methylcellulose; polyvinyl alcohol, and starch.

Bacterial hemicellulase can be used with a wide variety of watersoluble antimicrobial preservatives. 
However, Bacterial hemicellulase produces an immediate increase in viscosity when added to Bacterial hemicellulase solutions.

Bacterial hemicellulase has good tolerance for dissolved electrolytes, although it may be salted out of solution when mixed with certain salt solutions.
Bacterial hemicellulases will precipitate a 10% w/v solution of Cellosize WP-09 and a 2% w/v solution of Cellosize WP-4400: sodium carbonate 50% and saturated solutions of aluminum sulfate; ammonium sulfate; chromic sulfate; disodium phosphate; magnesium sulfate; potassium ferrocyanide; sodium sulfate; sodium sulfite; sodium thiosulfate; and zinc sulfate.

Bacterial hemicellulase is also incompatible with certain fluorescent dyes or optical brighteners, and certain quaternary disinfectants which will increase the viscosity of aqueous solutions.
Bacterial hemicellulase, 3,400 cps is a thickening and gelling agent created from cellulose, most commonly used in cleaning solutions, cosmetics and other products used in the household. 

Bacterial hemicellulase usually comes in capsule form. 
Ungraded products supplied are indicative of a grade suitable for general industrial use or research purposes and typically are not suitable for human consumption or therapeutic use.

Bacterial hemicellulase (HEC) polymer is a hydroxyethyl ether of cellulose, obtained by treating cellulose with sodium hydroxide and reacting with ethylene oxide. 
Bacterial hemicellulase polymers are largely used as water-binder and thickening agent in many industry applications, that is, personal care products, pharmaceutical formulations, building materials, adhesives, etc., and as stabilizer for liquid soaps. 

They are available as white free-flowing granular powders that easily dissolve in cold and hot water to give transparent solutions with varying viscosities depending on polymer concentration, type and temperature.
Bacterial hemicellulase acts as a demulcent by relieving inflammation or irritation and dryness of eyes.

Bacterial hemicellulase is a gelling and thickening agent that derives from cellulose (found in the cell walls of plants).
Bacterial hemicellulase is widely used in the cosmetics and hair industry due to its thickening properties and water solubility. 

Although Bacterial hemicellulase is water soluble, there are many factors that contribute to how it reacts when making a product. 
The most common grade of Bacterial hemicellulase used in the cosmetics industry is grade R, this is due to other grades resulting in lumping once they come in contact with water. 

The temperature as well as the pH levels in the water factor into how well it is able to dissolve. 
Bacterial hemicellulase can take anywhere from 4-25 minutes to dissolve once it has been entered into the liquid. 

Once dissolved, the product is thicker and much easier to apply to the skin.
Bacterial hemicellulase is used to treat medical conditions characterized by insufficient tear production such as keratoconjunctivitis sicca), recurrent corneal erosions, decreased corneal sensitivity, exposure and neuroparalytic keratitis. 

Bacterial hemicellulase is also used as a lubricant for artificial eyes. HPC is used as a thickener, a low level binder and as an emulsion stabiliser with E number E463. 
In pharmaceuticals Bacterial hemicellulase is used as a binder in tablets.

Bacterial hemicellulase is a chemical substance with the chemical formula C3H7O that is an ether of cellulose. 
Bacterial hemicellulase presents as a white to light cream powder. 

Bacterial hemicellulase is formed when some of the hydroxyl groups in the successive glucose units have been hydroxypropylated.
Bacterial hemicellulase is mostly used as a constituent in pharmaceutical products, and care must be taken to avoid the corrosion of process vessels during its production.

Bacterial hemicellulase is a cellulose ether containing hydroxypropyl substitution. 
Bacterial hemicellulase occurs as a white powder. 

Bacterial hemicellulase is soluble in water and in certain organic solvents. 
Bacterial hemicellulase may contain a suitable anticaking agent.

Bacterial hemicellulase is widely used in oral and topical pharmaceutical formulations.
Bacterial hemicellulase is primarily used in tableting as a binder,film-coating,and extended-release-matrix former. 

Concentrations of Bacterial hemicellulase of 2–6% w/w may be used as a binder in either wet-granulation or dry, direct compression tableting processes. 
Concentrations of 15–35% w/w of Bacterial hemicellulase may be used to produce tablets with an extended drug release. 

The release rate of a drug increases with decreasing viscosity of Bacterial hemicellulase. The addition of an anionic surfactant similarly increases the viscosity of Bacterial hemicellulase and hence decreases the release rate of a drug. 
Blends of Bacterial hemicellulase and other cellulosic polymers have been used to improve wet granulation characteristics and tableting characteristics, as well as to achieve better control and manipulation of the rate of drug release. 

As an alternative technology to wet granulation, dry granulation and direct compression of Bacterial hemicellulase formulations have been reported to exhibit acceptable tableting and flow characteristics for application in extended-release matrix tablets. 
Typically, a 5% w/w solution of Bacterial hemicellulase may be used to film-coat tablets. 

Aqueous solutions containing Bacterial hemicellulase together with an amount of methyl cellulose or ethanolic solutions have been used. Stearic acid or palmitic acid may be added to ethanolic Bacterial hemicellulase solutions as plasticizers. 
Environmental concerns have limited the use of ethanol in film coating solutions. A low-substituted Bacterial hemicellulase is used as a tablet disintegrant; see Bacterial hemicellulase, Low-substituted.

Bacterial hemicellulase is also used in microencapsulation processes and as a thickening agent. In topical formulations, Bacterial hemicellulase is used in transdermal patches and ophthalmic preparations.
Bacterial hemicellulase is also used in cosmetics and in food products as an emulsifier and stabilizer.

Bacterial hemicellulase is a derivative of cellulose that is soluble in both water and organic solvents. 
Bacterial hemicellulase is particularly good at trapping water and producing a film that serves as a barrier to water loss. 

Bacterial hemicellulase possesses good surface activity but does not gel as it forms open helical coils. 
In general Bacterial hemicellulase is a water-soluble thickener, emulsifier and film-former often used in tablet coating.

Bacterial hemicellulase is used as a sieving matrix for DNA separations by capillary and microchip electrophoresis.
Bacterial hemicellulase is the main ingredient in Cellugel, which is used in book conservation. 

Bacterial hemicellulase is described as "A safe, penetrating consolidant for leather book covers affected by red rot" and is produced by Preservation Solutions.
Bacterial hemicellulase (HPC) is a derivative of cellulose that gives rise to bright colours when in Bacterial hemicellulase's liquid crystalline phase and is responsive to external stimuli. 

Bacterial hemicellulase has the advantages of being biocompatible and edible and as such, commonly found as a thickening agent in pharmaceuticals and in food products.
The most common grade of Bacterial hemicellulase used in the cosmetics industry is grade R.

This is due to other grades resulting in lumping once they come in contact with water. 
The temperature as well as the pH levels in the water factor into how well it is able to dissolve. 

Bacterial hemicellulase can take anywhere from 4-25 minutes to dissolve once it has been entered into the liquid. 
Once dissolved, the product is thicker and much easier to apply to the skin.

Bacterial hemicellulase acts as one of the key ingredient and viscosity-enhancing agent to prolong corneal contact time and increase intraocular drug levels.
Bacterial hemicellulase is a polysaccharide derivative with gel thickening, emulsifying, bubble-forming, water-retaining and stabilizing properties. 

Bacterial hemicellulase is used as a key ingredient in many household cleaning products, lubricants and cosmetics due to its non-ionic and water-soluble nature. 
Bacterial hemicellulase is often used as an ingredient in ophthalmic pharmaceutical preparations such as artificial tear solutions and adjunct agent in topical drug formulations to facilitate the delivery of drugs with hydrophobic character.

Bacterial hemicellulase is a thickening or gelling agent made from cellulose. 
The non-ionic water-soluble polymer readily dissolves in cold or hot water.

Bacterial hemicellulase is used as a gelling and thickening agent in the development of biostructures for the delivery of hydrophobic drugs.
Bacterial hemicellulase is used in the development of polymer networks and block copolymers useful in separation technology such as capillary electrophoresis and in biofilms and coatings.

Bacterial hemicellulase is a non-ionic, water soluble polymer used as a thickening agent for aqueous cosmetic and personal care formulations. 
Bacterial hemicellulase will produce crystal clear gel products and thicken the aqueous phase of cosmetic emulsions. 

Bacterial hemicellulase can be also be used to efficiently thicken shampoos, body washes and shower gels.
One of the problems normally associated with this and other water-soluble thickeners is the tendency of the particles to agglomerate or lump when first wetted with water. 

The high-purity cosmetic grade of Hydroxyethylcellulose we offer is an R-grade, designed to be added to water without lumping, and thus greatly facilitating solution preparation. 
Bacterial hemicellulase is also the most efficient grade of non-ionic thickener available from the manufacturer.

Hydration of the R-grade particles has been inhibited.
When the particles are added to water, they disperse without lumping, and following a predetermined delay, begin to dissolve. 

This process permits the preparation of clear, smooth, viscous solutions in a short period of time by simply adding the R-grade to water and stirring until the polymer is completely dissolved to prevent settling of the particles.
The inhibition period, from the initial wetting to the start of dissolution, is referred to as the hydration time. 

This hydration time can vary from 4-25 min. 
Hydration time is markedly affected by two factors: pH and temperature of the water. 

A higher temperature and a higher pH decrease the hydration time, but a too high temperature or pH can result in lumping. 
So, it is recommended that Bacterial hemicellulase be added to room temperature water with a neutral pH. 

Bacterial hemicellulase can be heated and the pH can be adjusted as may be needed.
Bacterial hemicellulase is mainly used in water-based products. 

Bacterial hemicellulase finds applications as a binder, film former, rheology modifer (thickener), adhesion promoter, dispersion stabilizer, extender and slumping reducer in numerous products including paints, inks, adhesives, cosmetics, personal care products, textiles, cements, ceramics and paper products.
One of the most important applications of Bacterial hemicellulase and HMHEC are waterborne architectual coatings. 

They are either used alone or in combination with other thickeners. 
Infact, Bacterial hemicellulase is the most widely used thickener in exterior latex paints because it is compatible with many coating ingredients such as pigments, surfactants, emulsifiers, preservatives, and binders.

Bacterial hemicellulase (EHEC) is a non-ionic, water-soluble cellulose derivative produced by introduction of ethylene oxide groups to the hydroxyl groups of the cellulose backbone. 
Bacterial hemicellulase is useful as a water thickener, rheological control additive, protective colloid, binder, stabilizer, suspending agent and film former.  

Bacterial hemicellulase is used in many industrial applications including latex paints, emulsion polymerization, petroleum, paper, pharmaceuticals, cosmetics and many other applications.
Bacterial hemicellulase is a group of enzymes that catalyzes the breakdown of heteropolysaccharides. It includes glucanases that act on glucan. Similarly, xylanases and mannanases breakdown xylan and mannan respectively. Hemicellulase is prevalent among various bacteria, fungi and plants.

 


USES:

Bacterial hemicellulase is used for cracking method to extract polymerized dispersing agents such as oil water base gel fracturing fluid, polystyrene and polyvinyl chloride.
As surfactants, protective colloids, emulsion stabilizers in combination with emulsion such as vinyl chloride, vinyl acetate emulsion, and a tackifier, dispersant, dispersion stabilizer of emulsion. 

Widely used in many aspects such as coatings, fibers, dyeing, paper, cosmetics, pharmaceuticals, pesticides. 
There are many uses in oil exploitation and machinery industry.

As surfactants, latex thickening agent, protective colloid, oil exploitation fracturing fluid and polystyrene and polyvinyl chloride dispersing agents, etc.
Also for latex thickening agent in paint industry, hygristor in electronics industry, cement anti-coagulant agent and water retention agent in construction industry. 

Glazing in ceramic industry and toothpaste binder. 
Also widely used in many aspects such as printing and dyeing, textile, paper, pharmaceutical, health, food, cigarettes, pesticides and fire extinguishing agent.

 


APPLICATION:

This result in improved development and extensibility of gluten produced by these enzymes enhance the quality and flavour of flour. 
Bacterial hemicellulase is specialty used as an ingredient in flour improvement

Thickener, binder, and emulsifier.Bacterial hemicellulase is used as an emulsifier, stabilizer, whipping aid and thickener. 
Bacterial hemicellulase is also used as a binder in ceramics and glazes, additive in hair and cosmetic products and blow-molded bottles. 

Further, Bacterial hemicellulase is used in contact lens wetting solutions, as a formulation aid and texturizer for foods. 
In addition, Bacterial hemicellulase serves as a topical ophthalmic protectant and lubricant. 
Bacterial hemicellulase acts as a disintegrant and a binder in tablets. 

 

APPLICATION:

powder or liquid.

 

PROPERTIES:

-form: powder

-specific activity: 0.3-3.0 unit/mg solid (using a β-galactose dehydrogenase system and locust bean gum as substrate)

-greener alternative product characteristics: Waste Prevention, Design for Energy Efficiency

-greener alternative category: Enabling
 
-storage temp.:−20°C

 


SYNONYM:

Hemicellulase
Bacterial Hemicellulase enzyme 
for flour
Hemicellulase enzyme
development and extensibility of gluten enzyme 
gluten 
flour 
Bacterial Hemicellulase enzyme for flour

 

 

 

 

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