PRODUCTS

NIPACIDE CFX 3

Nipacide CFX 3 is a water-based low toxicity isothiazolinone/ formaldehyde donor biocide, developed for the complete in-can and head space protection of water-based products. Nipacide CFX 3 is effective against a wide range of microorganisms including gram positive and gram negative bacteria, yeast and fungi.

CAS No. : 55965-84-9

Synonyms:
formaldehyde + chloromethyl and methylisothiazolinone; formaldehyde-chloromethyl-methylisothiazolinone; Nipacide-CFX-3; Nipacide CFX3; nipacid cfx 3; nipasid cfx 3; nipasit cfx 3; Combination of 5 – Chloro – 2 – methyl - isothiazolin – 3 - one (CIT) / 2 – Methyl – isothiazolin – 3 - one (MIT) and 1,6 – dihydroxy - 2,5 – dioxahexane; Nipacide CFX/3; 5-chloro-2-methyl-2Hisothiazol-3-one and 2-methyl-2H-isothiazol-3-one; Con WT; Kathon 886; kathon biocide; CMIT/MIT 1.5CG; CMIT/MIT B/PLUS; CMI/MI Kathon 886; (96118-96-6) kathon 886; CIT/MIT14 Kathon Biocide; Mixture with methylchloroisothiazolinone; 5-Chloro-2-methyl-3(2H)-isothiazolone with 2-methyl-3(2H)-isothiazolone; 2-Octyl-4-isothiazolin-3-one; 3(2H)-Isothiazolone, 2-octyl-; Kathon LP Preservative; Octhilinone; Ultrafresh DM 25; Vinyzene IT 3000DIDP; Octhilinone; 26530-20-1; 2-n-Octyl-4-isothiazolin-3-one; 2-Octyl-4-isothiazolin-3-one; 2-octylisothiazol-3(2H)-one; 2-Octyl-3-isothiazolone; Pancil; 2-Octyl-2H-isothiazol-3-one; 3(2H)-Isothiazolone, 2-octyl-; Kathon 893; 2-Octyl-3(2H)-isothiazolone; Skane 8; Skane M-8; Kathon 893F; Micro-chek skane; Kathon LM; Microbicide M-8; 2-octyl-1,2-thiazol-3-one; Micro-chek 11; Micro-chek 11D; Kathon SP 70; Kathon LP preservative; Kathon 4200; Pancil-T; Skane M8; Caswell No. 613C; 4-Isothiazolin-3-one, 2-octyl-; UNII-4LFS24GD0V; Vinylzene IT 3000DIDP; Octyl-3(2H)-isothiazolone; RH 893; Octhilinone [ANSI:BSI:ISO]; CCRIS 6082; EINECS 247-761-7; 2-Octyl-3-isothioazolone; 2-Octyl-3-isothiazolinone; Octyl-4-isothiazol-3-one; EPA Pesticide Chemical Code 099901; 2-n-Octyl-3-isothiazolone; Kathon; CAS-26530-20-1; C11H19NOS; Pancil T; 2-octyl-1,2-thiazol-3(2H)-one

Nipacide CFX 3

Nipacide CFX 3 is an in-can biocide based on formaldehyde + chloromethyl and methylisothiazolinone.

When products are prone to surface contamination by fungi and head space protection is required, Nipacide CFX 3 should be the biocide of choice. Nipacide CFX 3 is effective against a wide range of spoilage organisms effective over a wide pH and temperature range.

Benefits of Nipacide CFX 3
provides heat-space protection
bactericide
fungicide
pH stable 4-9
temperature stable up to 40° C
FDA approved for various applications

Technical data of Nipacide CFX 3
Product class   Formaldehyde + chloromethyl and methylisothiazolinone
Physical Form   Solution
Active content (ca.)    23%
pH stability   4 - 9
Temperature stability    40°C
Recommended Use Level     0.075 - 0.2%

Applications of Nipacide CFX 3
Nipacide CFX 3 is recommended for preservation of a wide range of applications including:

water-based adhesives
polymer emulsions
water based decorative paints
metal working fluids
construction chemicals

Nipacide CFX 3 is a low toxicity, in-can biocide based on formaldehyde, chloromethyl and methylisothiazolinone. Nipacide CFX 3 is a water-based isothiazolinone/ formaldehyde donor biocide developed for complete head space protection of water-based products. Nipacide CFX 3 is effective against gram positive and gram negative bacteria, yeast and fungi over a wide pH and temperature range. Nipacide CFX 3 complies with FDA regulation.

Description
Nipacide CFX 3-IN is a water based, liquid biocide specifically developed for the complete in-can microbiological protection of industrial water based products against bacterial and fungal spoilage in the wet state.

Applications of Nipacide CFX 3
Nipacide CFX 3-IN is recommended for the preservation of a wide range of applications including water based adhesives, polymer emulsions, water based decorative paints, metal working fluids, sealants and tile grouts, household detergent cleaners, car care products and construction chemicals where protection against fungi and bacteria is required in the wet state. Nipacide CFX 3 is effective against a wide range of common spoilage organisms including gram positive and gram negative bacteria, yeast and fungi. Nipacide CFX 3 can be used over a pH range 4 - 9 and temperature range up to 40°C. Nipacide CFX 3-IN is particularly effective at providing head space protection, in-can, by the slow release of formaldehyde.

Microbiological Data of Nipacide CFX 3
Nipacide CFX 3-IN exhibits a broad spectrum of activity which is demonstrated by the following MIC data against some common microorganisms associated with wet-state spoilage:

Organism MIC (ppm) Organism MIC (ppm)
Bacteria: Fungi:
Pseudomonas aeruginosa 400 Aspergillus niger 150
Pseudomonas putida 400 Penicillium mineoluteum 75
Proteus vulgaris 125 Fusarium solani 125
Escherichia coli 500 Geotrichum candidum 200
Staphylococcus aureus 550 Yeast:
Candida albicans 150

Chemical Compatibility of Nipacide CFX 3
Nipacide CFX 3-IN is compatible with most raw materials used in the manufacture of industrial and decorative coatings. However it is recommended that the compatibility of Nipacide CFX 3-IN with the application should always be checked and evaluated before use.

Use Levels of Nipacide CFX 3
Nipacide CFX 3-IN should be evaluated in finished products at levels between 0.1% and 0.3%. Please note that Nipacide CFX 3-IN above 0.16% requires R43 (causes sensitization by skin contact) hazard labeling. The level of protection required will depend on many factors including the degree of contamination of raw materials and the susceptibility of the final product.

Applications of Nipacide CFX 3
Building & Construction — Building Materials
Decorative Paints & Coatings
Coating Type
Waterborne

Nipacide CFX 3 is a low toxicity biocide specifically developed for the complete microbiological protection of water based products against bacterial and fungal spoilage in the wet state, particularly where it is to be used at high ambient temperatures. This product is a water based liquid. Nipacide CFX 3 is described chemically as a blend of chlorinated and non-chlorinated isothiazolinones plus ethyl glycol bis hemiformal.

A unique stabilisation system has been developed and incorporated into Nipacide CFX/3. Nipacide CFX 3 is recommended for a wide range of applications including adhesives, polymer emulsions, fountain solutions, MWF and paint where protection against fungi and bacteria is required in the wet state.

Application of Nipacide CFX 3 :
Nipacide CFX3 is recommended for preservation of a wide range of applications including water based adhesives, polymer emulsions; water based decorative paints, metal working fluids and construction chemicals. When products are prone to surface contamination by fungi and head space protection is required, Nipacide CFX3 should be one of the biocides of choice. Nipacide CFX3 is effective against a wide range of spoilage organisms effective over a wide pH and temperature range.

Chemical Composition of Nipacide CFX 3
Blend of ethyl glycol bis hemiformal, chlorinated and non-chlorinated isothiazolinones

Advantages of Nipacide CFX 3
Low toxicity biocide
Effective against bacteria, fungi and yeast
Specifically developed for the complete microbiological protection of water based products against bacterial and fungal spoilage in the wet state.
Other Applications
Waterborne Coatings

Nipacide CFX 3 is a low toxicity biocide specifically developed for the complete microbiological protection of water based products against bacterial and fungal spoilage in the wet state. Nipacide CFX 3 is a glycol based solution. This yellow liquid is recommended for a wide range of applications including adhesives, polymer emulsions, fountain solutions, MWF and paint where protection against fungi and bacteria is required in the wet state.

Nipacide CFX 3 is an in-can biocide based on formaldehyde + chloromethyl and methylisothiazolinone. Nipacide CFX 3 is a low toxicity biocide, developed for the complete protection of water based products. Nipacide CFX 3 is effective against bacteria, yeast and fungi. Nipacide CFX 3 is recommended for a wide range of applications including preservation of paints and varnishes, adhesives, emulsions (polymer), cutting fluids, where protection against bacteria and fungi is required in the wet state (In can protection). Nipacide CFX 3 is stable in the temperature range of 5°C to 40 °C and in the range of pH 4 to 10.0.

Benefits of Nipacide CFX 3
Provides heat-space protection
Bactericide
Fungicide
pH stable 4-10
Temperature stable up to 40° C

Applications & Uses of Nipacide CFX 3
Markets
Building & Construction
Paints & Coatings

Nipacide CFX 3 is a low toxicity biocide, developed for the complete protection of water based products. Nipacide CFX 3 is effective against bacteria, yeast and fungi. Nipacide CFX 3 is recommended for a wide range of applications including preservation of paints and varnishes, adhesives, emulsions (polymer), cutting fluids, where protection against bacteria and fungi is required in the wet state (In can protection).

About Nipacide CFX 3

Nipacide CFX 3 is registered under the REACH Regulation and is manufactured in and / or imported to the European Economic Area, at ≥ 10 to < 100 tonnes per annum.

Nipacide CFX 3 is used by consumers, by professional workers (widespread uses), in formulation or re-packing and in manufacturing.

Consumer Uses of Nipacide CFX 3
Nipacide CFX 3 is used in the following products: cosmetics and personal care products, fertilisers, plant protection products and perfumes and fragrances.
Other release to the environment of Nipacide CFX 3 is likely to occur from: outdoor use as processing aid and indoor use as processing aid.

Article service life of Nipacide CFX 3
ECHA has no public registered data on the routes by which Nipacide CFX 3 is most likely to be released to the environment. ECHA has no public registered data indicating whether or into which articles the substance might have been processed.

Widespread uses by professional workers of Nipacide CFX 3
Nipacide CFX 3 is used in the following products: fertilisers, plant protection products, laboratory chemicals, perfumes and fragrances and cosmetics and personal care products. Nipacide CFX 3 is used in the following areas: agriculture, forestry and fishing. Other release to the environment of Nipacide CFX 3 is likely to occur from: outdoor use as processing aid and indoor use as processing aid.

Formulation or re-packing of Nipacide CFX 3
Nipacide CFX 3 is used in the following products: laboratory chemicals, fertilisers, plant protection products, cosmetics and personal care products, perfumes and fragrances and biocides (e.g. disinfectants, pest control products).
Release to the environment of Nipacide CFX 3 can occur from industrial use: formulation of mixtures and formulation in materials.

Uses at industrial sites of Nipacide CFX 3
ECHA has no public registered data indicating whether or in which chemical products the substance might be used. ECHA has no public registered data on the types of manufacture using Nipacide CFX 3. ECHA has no public registered data on the routes by which Nipacide CFX 3 is most likely to be released to the environment.

Manufacture of Nipacide CFX 3
Release to the environment of Nipacide CFX 3 can occur from industrial use: manufacturing of the substance.

Compatibility of Nipacide CFX 3
In concentrate and use-dilution metalworking fluids, the compatibility of Nipacide CFX 3 Biocide is concentration-dependent and varies from formulation to formulation. It is compatible with most metalworking fluid additives, including surfactants and amines. Compatibility with amines may vary by the type, concentration and pH. Strong reducing agents, such as sulfides, mercaptans, bisulfites and metabisulfites, or strong oxidizing agents, such as hypochlorites, may affect the efficacy of Nipacide CFX 3. Laboratory or small-scale tests are recommended in order to evaluate Nipacide CFX 3 compatibility in use-dilution or concentrate metalworking fluids prior to commercialization.

Stability of Nipacide CFX 3
In-Use Stability: Nipacide CFX 3 Biocide has excellent stability in end use dilutions of metalworking fluids. It is stable over a wide pH range (4-10) in water and in metalworking fluid systems.
Concentrate Stability: Nipacide CFX 3 Biocide stability, in metalworking fluid concentrates, is variable. We recommend checking stability and performance before commercialization of products. Dow has several recommended stabilizers to improve stability and compatibility in many types of concentrates.
Storage Stability: In general, the storage stability of the Nipacide CFX 3 Biocide product is excellent. The shelf life of the product is nominally twelve years at 25°C. It is strongly recommended, however, that both the stability and compatibility of Nipacide CFX 3 Biocide in metalworking fluid formulations or systems be thoroughly examined before commercialization.

Method of Addition of Nipacide CFX 3
Nipacide CFX 3 Biocide should be directly dispensed into metalworking fluid concentrates or use-dilution metalworking fluids using a metering pump or other point-of-use device where possible and uniformLy dispersed throughout the fluid.

Fluid Concentrate of Nipacide CFX 3
Nipacide CFX 3 Biocide should be added to metalworking fluid concentrates at a level that ensures the final use-dilution fluid will contain 55 to 167 ppm of product (25 to 75 ppm active ingredient). Nipacide CFX 3 stability in a given concentrate should be determined prior to commercialization. Contact your local Dow representative for assistance in selecting one of several recommended stabilizers to enhance the performance and compatibility of Nipacide CFX 3 in your metalworking fluid concentrate.

Use-Dilution Fluid
We highly recommend grossly contaminated systems be cleaned before treatment is begun.
Initial Dose: For a noticeably fouled system, add 0.47 to 1.44 lbs (7 to 21 fl oz) of Nipacide CFX 3 Biocide per 1,000 gallons of fluid. This will provide 25 to 75 ppm active ingredient. Repeat until control is achieved.
Subsequent Dose: For maintenance of a non-fouled system, add 0.09 to 0.58 lbs (1.3 to 8.6 fl oz) of Nipacide CFX 3 Biocide per 1,000 gallons of fluid every four weeks. This will provide 5 to 30 ppm active ingredient. A higher dose range and/or increased frequency of treatment may be required, depending upon the rate of dilution of the preservative with the makeup fluid, the nature and severity of contamination, level of control required, filtration effectiveness, system design, etc.

Nipacide CFX 3 Biocide utilizes a two-step mechanism involving rapid growth inhibition leading to a loss of cell viability. Growth inhibition is the result of rapid disruption of the central metabolic pathways of the cell by inhibition of several specific enzymes, including dehydrogenases. The critical enzymes which are affected are associated with the Krebs cycle, nutrient metabolism and energy generation. The key physiological activities that are rapidly inhibited in microbial cells are respiration (oxygen consumption), energy generation (ATP synthesis), and growth (assimilation). Many of these key enzymes are present in both aerobic and anaerobic microorganisms, which explains why Nipacide CFX 3 is such a broad spectrum biocide.

Inhibition of cellular activity and growth is rapid (within minutes), whereas cell death (cidal activity) is observed after several hours’ contact. In general, the higher the concentration of biocide, the shorter the contact time required for more complete kill. Cell death results from the progressive loss of protein thiols in the cell from one of multiple pathways. As cell metabolism is disrupted, free radicals are produced which also results in cell death. This unique mechanism results in the broad spectrum of activity of Nipacide CFX 3 Biocide, low use levels for microbial control, and difficulty in attaining resistance by mutation. See technical bulletin (CS-632) for more detailed information.

How Rapidly Does Nipacide CFX 3 Biocide Work?
Within minutes after addition of Nipacide CFX 3 Biocide to a metalworking fluid sump, the metabolic activity of the microorganisms in the system shuts down. This includes cellular respiration (oxygen uptake), growth, energy generation, and nutrient uptake. The microorganisms, although still alive, are no longer able to reproduce or metabolize metalworking fluid components. After 24 to 48 hours of contact with a lethal dose of the biocide, most of the microorganisms have been killed.

How Long Does Nipacide CFX 3 Biocide Last?
Nipacide CFX 3 Biocide has excellent in-use stability and generally retains its antimicrobial efficacy in metalworking fluid systems for 2 to 4 weeks. Variables such as degree of fluid contamination, effectiveness of the filtration system, system turnover time, compatibility between the microbicide and the metalworking fluid components, and other system additives involved, can affect the life of the microbicide in a system.

Is Nipacide CFX 3 Biocide Effective in Reducing Fungal Biofilms?
YES. Nipacide CFX 3 Biocide has been shown to reduce microbial fouling and prevent biofilm development in metalworking fluid systems. The benefits of reduced fungal biofouling include improved system performance, reduced filter plugging, reduced biocorrosion, and improved microbial control.

Is Nipacide CFX 3 Biocide Effective When Used in Concentrates?
YES. Nipacide CFX 3 Biocide may be used in certain fluid concentrates to provide efficacy in the final use dilutions. Although Nipacide CFX 3 stability may not be suitable for all concentrates, we have had success with the biocide alone or in combination with one of our recommended stabilizers.

How Can I Improve Nipacide CFX 3 Biocide Stability in Concentrates?
We recommend testing Nipacide CFX 3 Biocide in concentrates prior to commercialization. Dow technical staff can assist you in formulating products. We have years of experience and a range of recommended stabilizers to prolong the lifetime and improve compatibility of Nipacide CFX 3 in concentrates. Contact your sales representative for assistance.

Initial determinations of the efficacy of any biocidal product are made via minimum inhibitory concentration (MIC) measurements. The MIC test yields valuable information about the product’s inherent antimicrobial efficacy and spectrum of activity. The MIC for any product is the lowest level at which the active ingredient inhibits the growth of various microorganisms. This method is a useful tool for screening antimicrobial agents under standardized laboratory conditions, in nutrient-rich growth conditions. In interpreting the data, remember that low values correspond to high activity. Table 2 indicates that Nipacide CFX 3 Biocide possesses outstanding antimicrobial activity against a broad range of fungi (both yeasts and molds). Nipacide CFX 3 has very low MIC values for most of the fungi tested and there is no gap in the spectrum of activity among the organisms tested.

Nipacide CFX 3 Biocide was evaluated as a tankside fungicide in a wide variety of metalworking fluids, including synthetics, semi-synthetics, and soluble-oil fluids. In a oneweek eradication study described below, a total of 16 fluids from various manufacturers in the United States, Europe, and Japan were tested.

Test Procedure
The actual test systems were run in volumes of 50 mL, which consisted of 40 mL of virgin metalworking fluid (generally diluted 20:1) and 10 mL of the adapted inoculum as described above. Prior to inoculation, the fluids containing fungal growth were blended for two minutes at high speed in a Waring blender. Most samples contained 0.5 g of iron filings. At time zero, the following active levels of Nipacide CFX 3 Biocide were added: 5 ppm, 10 ppm, 25 ppm, 50 ppm, 75 ppm, and 100 ppm. Additionally, samples were run containing 50 ppm and 100 ppm active sodium Pyrithione. Once fluids were dosed with biocide and inoculated, they were mechanically shaken for one week and plated on sabouraud dextrose agar.

Results
Nipacide CFX 3 Biocide was completely effective in all fluids at levels ranging from 5 to 75 ppm active ingredient. In all but one of the fluids, it was effective at concentrations in the range of 5 to 50 ppm active ingredient. In synthetic fluids, which are prone to fungal growth, Nipacide CFX 3 was effective in the range of 5 to 10 ppm. Sodium Pyrithione was not very effective at recommended use levels of 50 to 100 ppm active ingredient. A long-term study was done to compare the fungicidal activity of Nipacide CFX 3 Biocide and sodium Pyrithione in a synthetic metalworking fluid, use-dilution 1:30. The concentration of Nipacide CFX 3 studied ranged from 10 to 75 ppm active ingredient and the concentration of sodium pyrithione ranged from 50 to 200 ppm active ingredient.

Test Procedure
The test samples were inoculated at zero time and again every two weeks with fungal inoculum isolated from naturally contaminated synthetic metalworking fluid and maintained in the same fluid employed in the test.

Results
Results, provided in Table 4, show that particularly high fungal counts were not achieved in the untreated control for this particular fluid (note: Due to the inherent mycelial clumping common to most fungal species when growing in liquid substrates, plate counts of colonyforming units carried out on the aliquots of the liquid are not always indicative of the degree of fungal contamination present). In spite of this, sodium Pyrithione allowed fungal survival at all levels at which it was tested. Nipacide CFX 3 Biocide, however, exhibited complete fungal control at significantly lower levels.

During the first 45 days of the trial, the level of Nipacide CFX 3 Biocide was maintained at approximately 25 ppm active ingredient. For the remaining 3 months of the trial, the level of Nipacide CFX 3 was maintained between 30 ppm and 10 ppm active ingredient. The results of the trial showed that the regimen of Nipacide CFX 3 addition chosen provided essentially complete control of fungal organisms in the fluid itself and also destroyed the fungal organisms comprising the mats covering the walls of the flumes and weirs of the system. These fungal mats lost their integrity and gradually sloughed off the surfaces to which they were attached (see Figure 2). The microbial slime present on and around the machines also disappeared. The bacterial populations of the fluid remained in the range of 102 to 104 cfu/mL, throughout the trial. In addition, the amount of makeup fluid required to maintain the desired characteristics of the fluid was reduced significantly (42 percent) during the trial.

Handling
The procedures used for handling concentrated biocide solutions are similar to those used for handling concentrated acids and alkalis. The purpose is to prevent all eye and skin contact, including inhalation of mists, and thereby prevent possible injury and sensitization. Personnel handling Nipacide CFX 3 Biocide as supplied should always wear protective clothing, which includes chemical splash goggles, an impervious apron or rain suit, and impervious rubber gloves. We recommend that employees working with Nipacide CFX 3 as supplied thoroughly wash with soap and water at the end of a shift or prior to eating, drinking, smoking, or applying cosmetics. Special care should be taken to avoid contamination of surfaces or materials that may later be handled by unprotected personnel, for example, door and tap handles.

Storage
Nipacide CFX 3 Biocide is packaged in polyethylene or polyethylene-lined containers. It should not be stored in unlined metal containers since it is a corrosive material. Normal recommended storage temperatures are in the range of 10° to 25°C (50° to 80°F). Shelf life ~12 years (packaging should be evaluated and replaced as needed for transport compliance over the duration of product shelf life). Storage at >120°F for extended periods of time can result in degradation of the active ingredient.

Decontamination Solutions
Nipacide CFX 3 Biocide can be decontaminated with a 5% solution of sodium hypochlorite (NaOCl) containing 2-5% sodium bicarbonate (NaHCO3 ). Solutions should be freshly prepared. Employees preparing or handling decontamination solutions should wear chemical splash goggles, an impervious apron or rain suit, and impervious rubber gloves.
Note: Do not use decontamination solution to treat skin, eyes or clothing which have come in contact with Nipacide CFX 3.

Decontamination of Equipment
Equipment used in the handling of Nipacide CFX 3 Biocide, such as mix tanks, lines, pumps, etc., must be decontaminated before carrying out maintenance or used for other service. To decontaminate this equipment, estimate the volume of Nipacide CFX 3 remaining in the well-drained system. Prepare 10 volumes of decontamination solution per volume of Nipacide CFX 3 (45%) and circulate the mixture throughout the equipment. Be certain that the Nipacide CFX 3 and decontamination solution mix well. Wait at least 30 minutes to ensure complete reaction. Drain and rinse with clean water or detergent solution. Decontamination solution runoffs should be drained to a chemical sewer unless prohibited by state or local regulations. Drips, minor spills and exposed wet areas should be cleaned up promptly with the hypochlorite/bicarbonate mixture. Contaminated surfaces should be swabbed with decontamination solution and allowed to stand for 30 minutes before rinsing thoroughly with water. Decontaminated solutions should be drained to a chemical sewer unless prohibited by state or local regulations.