O-PHTHALALDEHYDE

O-Phthalaldehyde is a dialdehyde in which two formyl groups are attached to adjacent carbon centres on a benzene ring. It has a role as an epitope. It is a dialdehyde and a member of benzaldehydes. O-Phthalaldehyde is mainly used as a high-level disinfectant (a low-temperature chemical method) for heat-sensitive medical and dental equipment such as endoscopes and thermometers; in recent years, O-Phthalaldehyde has gained popularity as a safe and better alternative to glutaraldehyde.

CAS NO: 643-79-8

SYNONYM:
643-79-8; PHTHALALDEHYDE; o-Phthaldialdehyde; 1,2-Benzenedicarboxaldehyde; Benzene-1,2-dicarboxaldehyde; Phthaldialdehyde; Phthalic aldehyde; Phthalic dialdehyde; ortho-Phthalaldehyde; Phthalyldicarboxaldehyde; Phthalic dicarboxaldehyde; o-Phthaldehyde; benzene-1,2-dicarbaldehyde; Phthalaldialdehyde; o-Phthalicdicarboxaldehyde; 1,2-Diformylbenzene; 2-PHTHALALDEHYDE; 1,2-Phthalic dicarboxaldehyde; ortho Phthalaldehyde; o-Phthalic dicarboxaldehyde; 1,2-BENZENEDICARBALDEHYDE; OPA; OPTA; Phtalaldehydes; MFCD00003335; NSC 13394; UNII-4P8QP9768A; CHEBI:70851; 2-PHTHALDIALDEHYDE; EINECS 211-402-2; BRN 0878317; 4P8QP9768A; NCGC00166206-01; DSSTox_CID_12514; DSSTox_RID_78962; DSSTox_GSID_32514; Phtalaldehydes;1,2-Phthalic dicarboxaldehyde, 98+%; CAS-643-79-8; Orthophthaldialdehyde; ortho-Phthalic Aldehyd; phthalaldehyd; o-Phthalaldehyd; o-phthal aldehyde

There are some researches show, pH7.5 contains the sterilizing agent of O-Phthalaldehyde 0.5%, and its sterilizing power, sterilization speed, stability and toxicity all are better than glutaraldehyde, can kill mycobacterium in the 5min, the bacterium number reduces by 5 logarithmic value, and O-Phthalaldehyde is very stable, tasteless in pH3～9 scopes, non-stimulated to human nose, eye mucosa, and need not activate before using, various materials are had good consistency, have tangible microbiocidal activity.O-Phthalaldehyde can be widely used for precolumn derivatization of amino acids in HPLC separation or Capillary electrophoresis. For flow cytometric measurements of protein thiol groups. O-Phthalaldehyde can be used for precolumn derivatization of amino acids for HPLC separation and for flow cytometric measurements of protein thiol groups. Precolumn derivatization reagent for primary amines and amino acids. The fluorescent derivative can be detected by reverse-phase HPLC. The reaction requires O-Phthalaldehyde , primary amine and a sulfhydryl. In the presence of excess sulfhydryl, amines can be quantitated. In the presence of excess amine, sulfhydryls can be quantitated.

O-Phthalaldehyde is a high-level chemical disinfectant that is commonly used for disinfection of dental and medical instruments as an alternative to glutaraldehyde, which is a known skin and respiratory sensitizer. A variety of processes for manufacturing O-Phthalaldehyde have been reported in the literature. O-Phthalaldehyde is produced by heating pure benzaldehyde and chloroform with potassium hydroxide solution. The resulting solution is further acidified with hydrochloric acid and cooled to yield a colorless powder of o-phthalaldehyde. O-Phthalaldehyde is also produced by ozonization of naphthalene in alcohol followed by catalytic hydrogenation. Catalytic oxidation of various chemicals is also used in manufacturing o-phthalaldehyde. O-Phthalaldehyde can be manufactured by oxidation of phthalan by nitrogen monoxide in acetonitrile with N-hydroxyphthalimide as the catalyst to yield 80% to 90%. O-phthalaldehyde(OPA) is used for precolumn derivatization of amino acids for HPLC separation and for flow cytometric measurements of protein thiol groups. Used for fluorometric determination of histamine, histidine and other amino acids. Also O-Phthalaldehyde is  used for cholesterol assay in the picomole range.

Phthaldialdehyde has been used; in the preparation of O-phthaldialdehyde reagent for analysing gentamycin content, in the preparation of reagent for determining the degree of hydrolysis of milk proteins, in the measurement of free amino acids of milk samples by o-phthaldialdehyde/N-acetyl-L-cysteine (OPA/NAC) assay, in the derivatization of putrescine samples. The primary routes of human exposure to O-Phthalaldehyde are by inhalation and through the skin, which may occur through accidental or occupational exposures. Along with its increasing popularity as a chemical sterilizer, O-Phthalaldehyde has many applications in analytical methods and in diagnostic kits. O-Phthalaldehyde is also used as an intermediate in the synthesis of pharmaceuticals and as a reagent in the tanning industry, hair colorings, wood treatment, and antifouling paints. O-Phthalaldehyde was approved for use as an indoor antimicrobial pesticide in 1997; however, O-Phthalaldehyde is no longer registered with the United States Environmental Protection Agency (USEPA) for this use.

Phthalaldehyde (sometimes also o-phthalaldehyde or ortho-phthalaldehyde, Ortho phthalaldehyde) is the chemical compound with the formula C6H4(CHO)2. O-Phthalaldehyde is one of three isomers of benzene dicarbaldehyde, related to phthalic acid. This pale yellow solid is a building block in the synthesis of heterocyclic compounds and a reagent in the analysis of amino acids. Ortho phthalaldehyde dissolves in water solution at pH < 11.5. Its solutions degrade upon UV illumination and exposure to air. Ortho phthalaldehyde  was tested against a range of organisms including glutaraldehyde-resistant mycobacteria, Bacillus subtilis spores and coat-defective spores. Glutaraldehyde (GTA) and peracetic acid (PAA) were tested for comparative purposes. Both suspension and carrier tests were performed using a range of concentrations and exposure times. All three biocides were very effective (> or = 5 log reduction) against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa in suspension tests. Ortho phthalaldehyde and GTA (PAA was not tested) were also very effective against Staph. aureus and Ps. aeruginosa in carrier tests.

Ortho phthalaldehyde showed good activity against the mycobacteria tested including the two GTA-resistant strains, but 0.5% w/v Ortho phthalaldehyde was found not to be sporicidal. However, limited activity was found with higher concentrations and pH values. Coat-defective spores were more susceptible to Ortho phthalaldehyde, suggesting that the coat may be responsible for this resistance. The findings of this study suggest that Ortho phthalaldehyde is effective against GTA-resistant mycobacteria and that O-Phthalaldehyde is a viable alternative to GTA for high level disinfection. Ortho phthalaldehyde is used in a very sensitive fluorescent reagent for assaying amines or sulfhydryls in solution, notably contained in proteins, peptides, and amino acids, by capillary electrophoresis and chromatography. Ortho phthalaldehyde reacts specifically with primary amines above their isoelectric point Pi in presence of thiols.

Ortho phthalaldehyde reacts also with thiols in presence of an amine such as n-propylamine or 2-aminoethanol. The method is spectrometric (fluorescent emission at 436-475 nm (max 455 nm) with excitation at 330-390 nm (max. 340 nm)). Ortho phthalaldehyde is commonly used as a high-level disinfectant for medical instruments, commonly sold under the brand names of Cidex Ortho phthalaldehyde or TD-8. Disinfection with Ortho phthalaldehyde is indicated for semi-critical instruments that come into contact with mucous membranes or broken skin, such as specula, laryngeal mirrors, and internal ultrasound probes. Ortho phthalaldehyde can be polymerized. In the polymer, one of the oxygen atoms forms a bridge to the other non-ring carbon of the same phthalaldehyde unit, while the other bridges to a non-ring carbon of another phthalaldehyde unit. Poly(phthalaldehyde) is used in making a photoresist.
Ortho phthalaldehyde is a pale, yellow crystal or colorless powder. Ortho phthalaldehyde is soluble in water. Ortho phthalaldehyde is used as a disinfectant, mainly for dental and medical equipment.  Workers that produce or use Ortho phthalaldehyde may have direct skin contact.

The general population may be exposed by contact with residual disinfectant. If Ortho phthalaldehyde is released to the environment,  will be broken down in air by reaction with hydroxyl radicals. Ortho phthalaldehyde  may be broken down in the air by sunlight. Ortho phthalaldehyde  will not volatilize into air from soil or water surfaces. Ortho phthalaldehyde is expected to move easily through soil. Ortho phthalaldehyde is not expected to build up in fish. Irritation to the skin, eyes, and respiratory tract as well as asthma and allergic skin rashes have been reported in some healthcare workers that routinely use Ortho phthalaldehyde to disinfect equipment. Severe anaphylactic allergic reactions have been reported in some patients exposed to equipment disinfected with Ortho phthalaldehyde. Discoloration of the mouth and throat, burning of the throat, nausea, vomiting, and diarrhea may occur with ingestion. Damage to the nose, throat, lung, skin, and eyes were observed in laboratory animals following repeated exposure to low air levels of Ortho phthalaldehyde, damage was severe at moderate air levels and some animals died. Several alterations in the blood were also observed. Damage to the gastrointestinal tract, irregular breathing, impaired movement, and changes in the blood were observed in laboratory animals given moderate oral doses.

Some animals died at high oral doses. No evidence of abortion or birth defects were noted in laboratory animals exposed to Ortho phthalaldehyde during pregnancy, but delayed bone development was observed at high doses that made the mothers sick. Data on the potential for Ortho phthalaldehyde to cause infertility in laboratory animals were not available. However, damage to the testis and reduced sperm counts and motility were observed in male animals following repeated exposure to low air levels of Ortho phthalaldehyde. Data on the potential for Ortho phthalaldehyde to cause cancer in laboratory animals were not available. The potential for Ortho phthalaldehyde to cause cancer in humans has not been assessed by the U.S. EPA IRIS program, the International Agency for Research on Cancer, or the U.S. National Toxicology Program 14th Report on Carcinogens. Ortho Phthalaldehyde (Ortho phthalaldehyde) is used as disinfectant and reagent in fluorometric determination of primary amines and thiols.  Ortho phthalaldehyde is a commonly used solution for rapid sterilization of medical equipment. Cases of anaphylaxis following cystoscopy with endoscopes sterilized with this agent have been reported.

Ortho phthalaldehyde-induced anaphylaxis following laryngoscopy have also been described. In these patients, Ortho phthalaldehyde-specific IgE was detected in the serum. Contact dermatitis occurred in 4 workers of the endoscopy unit, one of whom also developed asthma. Among 80 female disinfection workers who used only antiseptic solutions containing Ortho phthalaldehyde, the incidence of disinfection-related complaints were 10% skin, 9% eye, and 16% respiratory symptoms. In male mice, injected Ortho phthalaldehyde induced specific IgE and IgG in the sera, suggesting that Ortho phthalaldehyde acts as a hapten. Overall, Ortho phthalaldehyde caused acute inflammation and acted as a haptenic allergen, although it caused only mild liver injury. In mice sensitized to ovalbumin , Ortho phthalaldehyde enhanced the induced recruitment of neutrophils to the lung and the production of allergen-specific IgE, suggesting that Ortho phthalaldehyde acts as an immunological adjuvant. The major targets from Ortho phthalaldehyde exposure in rats and mice included the respiratory system (nasal cavity, larynx, trachea, and lung), skin, eye, testis, and epididymis. The most sensitive measure of Ortho phthalaldehyde inhalation toxicity in male and female rats and mice was significantly increased incidences of nasal cavity lesions (lowest-observable-effect concentration = 0.44 ppm).

Ortho phthalaldehyde was mutagenic in Salmonella typhimurium strain TA100 in the absence of exogenous metabolic activation; no mutagenicity was seen in TA100 with metabolic activation or in TA98 or Escherichia coli WP2 uvrA/pKM101, with or without metabolic activation.  Ortho Phthalaldehyde's production and use as a disinfectant may result in its release to the environment through various waste streams. If released to air, a vapor pressure of 5.2X10-3 mm Hg at 21 °C indicates Ortho Phthalaldehyde will exist solely as a vapor in the atmosphere. Vapor-phase Ortho Phthalaldehyde will be degraded in the atmosphere by reaction with photochemically-produced hydroxyl radicals; the half-life for this reaction in air is estimated to be 17 hours. Ortho Phthalaldehyde has an absorption peak at 297 nm and, therefore, may be susceptible to direct photolysis by sunlight. If released to soil, Ortho Phthalaldehyde is expected to have very high mobility based upon an estimated Koc of 10. Volatilization from moist soil surfaces is not expected based upon an estimated Henry's Law constant of 1.8X10-8 atm-cu m/mole.

Ortho Phthalaldehyde is not expected to volatilize from dry soil surfaces based upon its vapor pressure. Biodegradation data in soil or water were not available. If released into water, Ortho Phthalaldehyde is not expected to adsorb to suspended solids and sediment based upon the estimated Koc. Volatilization from water surfaces is not expected to be an important fate process based upon this compound's estimated Henry's Law constant. An estimated BCF of 3 suggests the potential for bioconcentration in aquatic organisms is low. Hydrolysis is not expected to be an important environmental fate process since this compound lacks functional groups that hydrolyze under environmental conditions (pH 5 to 9). Occupational exposure to Ortho Phthalaldehyde may occur through dermal contact with this compound at workplaces where Ortho Phthalaldehyde is produced or used. Monitoring and use data indicate that the general population may be exposed to Ortho Phthalaldehyde via contact with products containing residual disinfectant. Because body fluids and blood have a tendency to adhere to transesophageal echo devices, a high level of sterilization is required when cleaning them.

Ortho phthalaldehyde has been widely used in Japan since being approved as a high-level sterilant. The authors report a patient with widespread, severe skin and mucous membrane damage of the lip, tongue, pharynx and esophagus areas that was attributed to inadequate washing after the sterilization of a transesophageal echo device with Ortho phthalaldehyde. This patient experienced sequelae, which did not improve after more than 1 year of continuous treatment. When using medical devices sterilized with Ortho phthalaldehyde, the use of a probe cover, when applicable, is recommended and complete washing prior to use is required.
 Orthophthalaldehyde (ortho phthal aldehyde) (orthophital aldehyde) (orthophthalaldehyde) (orthophital aldehyde) (ortho phthalaldehyde) (orthophthal aldehyde) (ortho phthal aldehyde) (Ortho phthal aldehyde) (Ortho Phthalaldehyde) (Phthalaldehyde) (Phthalaldehyde) Orthophthalaldehyde) (OrthoPhthalaldehyde) (ortho phthalaldehyde) (orthophital aldehyde) (orthophthalaldehyde) (orthophthaldehyde) (ortho phthalaldehyde) (orthophthal aldehyde) (ortho phthal aldehyde) (ortho phthal aldehyde) (OPA) with high disinfectant level (OPA) approved by the solus. Contains 1,2 Benzenedicarboxaldehyde. (Ortho Phthalaldehyde) (O- Phthalaldehyde) (Ortho-Phthalaldehyde) (Ortho-phthalaldehyde) (ortho phthalaldehyde) (orthophtaldehyde) (orthophthalaldehyde) (orthophtaldehyde) (ortho phthalaldehyde) (ortho phthalaldehyde) (ortho phthalaldehyde) ) (OrthoPhthalaldehyde) OPA is stable over a wide pH range (pH 3-9). It does not cause severe irritation on the eyes and respiratory tract mucosa, it does not require monitoring after exposure, it does not have an irritating odor.

It does not need to be activated. (Ortho Phthalaldehyde) (ortho phthalaldehyde) (orthophital aldehyde) (orthophthalaldehyde) (orthophthaldehyde) (ortho phthalaldehyde) (orthophthal aldehyde) (ortho phthal aldehyde) (O- Phthalaldehydehyde) ) (OrthoPhthalaldehyde) The disadvantage of OPA solution is that it stains proteins gray. Causes color change in poorly cleaned instruments. If applied to poorly cleaned medical equipment, it causes tissue fixation and biofilm formation. Personal protective equipment should be used during contact. In addition, the instruments should be thoroughly rinsed to prevent staining on the patient's mucosa or skin. (Ortho Phthalaldehyde) (ortho phthalaldehyde) (orthophital aldehyde) (orthophthalaldehyde) (orthophthaldehyde) (ortho phthalaldehyde) (orthophthal aldehyde) (ortho phthal aldehyde) (O- Phthalaldehydehyde) ) (OrthoPhthalaldehyde) OPA solution high-level disinfection time is 5-12 minutes. (5 minutes in Europe, Asia and Latin America countries. 10 minutes in Canada and Australia, 12 minutes in the United States) (Ortho Phthalaldehyde) (Ortho Phthalaldehyde) (ortho phthalaldehyde) (orthophthalaldehyde) ( orthophital aldehyde) (ortho phthalaldehyde) (orthophthal aldehyde) (ortho phthal aldehyde) (O- Phthalaldehyde) (Ortho-Phthalaldehyde) (OrthoPhthalaldehyde) (OrthoPhthalaldehyde) OPA should be disposed of in line with the current hazardous chemical waste disposal regulations and recommendations.

(Sporicidal) compounds used for high level disinfection High level (sporicidal) disinfectants and general properties Glutaraldehyde is widely used as high level disinfectant. Alkali glutaraldehyde kills vegetative bacteria at 2% density in 2 minutes, mycobacteria in 20 minutes, and spore bacteria in only 3 hours. A contact time of 20 minutes at room temperature is sufficient for high-level disinfection. It is preferred because it does not damage tools and is cheap. Since it is toxic and irritant, it should only be used in a well ventilated place and in closed containers. Their solutions can be used for 14-28 days. 10 minutes contact time with orthophthalaldehyde is sufficient for high-level disinfection. Orthophthalaldehyde (Ortho Phthalaldehyde) (Ortho Phthalaldehyde) (O- Phthalaldehyde) (Ortho-Phthalaldehyde) (ortho phthalaldehyde) (orthophtaldehyde) (orthophthalaldehyde) (orthophtaldehyde) (ortho phthalaldehyde) (orthodehydehyde) (orthodehydehyde) OrthoFitalaldehyde) (OrthoPhthalaldehyde) (OPA): It is a compound with less toxicity than glutaraldehyde and faster effective against mycobacteria. More expensive than glutaraldehyde.

However, both show protein fixing properties. For this reason, they should never be used in unclean conditions. A minimum of 6 hours is required for sterilization. Their solutions can be used for a maximum of 14 days. (Ortho Phthalaldehyde) (ortho phthalaldehyde) (orthophital aldehyde) (orthophthalaldehyde) (orthophital aldehyde) (ortho phthal aldehyde) (orthophthal aldehyde) (ortho phthal aldehyde) (Ortho Phthalaldehyde) (O- Phthalaldehyde) ) (OrthoPhthalaldehyde) As for glutaraldehyde, safety precautions should be taken for OPA, it should be used in a well ventilated place and in closed containers. (ortho phthal aldehyde) (orthophital aldehyde) (orthophthalaldehyde) (orthophital aldehyde) (orthophital aldehyde) (orthophthal aldehyde) (ortho phthal aldehyde) OPA (OrthoFitalaldehyde) (OrthoPhthalaldehyde) (Orthohythalaldehyde) (Phthahythalaldehyde) (Ortho-Phthalaldehyde) causes staining on contact surfaces, skin and clothes.

IUPAC NAME:
1,2-Benzenedicarboxaldehyde; Aldehyde, ortho-Phthalico Phthalaldehydeo Phthaldialdehydeo-Phthalaldehydeo-Phthaldialdehydeortho Phthalaldehydeortho Phthalic Aldehydeortho-Phthalaldehydeortho-Phthalic AldehydeOrthophthaldialdehyde; benzene-1,2-dicarbaldehyde; o-Phthalaldehyde; o-Phthalaldehyde; Phthalaldehyde; phthalaldehyde; Phthaldialdehyd

TRADE NAME:
Phthalaldialdehyde; Phthaldialdehyde; Phthalic aldehyde; Phthalic dialdehyde; Phthalic dicarboxaldehyde; Phthalyldicarboxaldehyde

OTHER NAME :
643-79-8

Orthophthalaldehyde is 1,2 benzene dicarboxialdehyde. It has the feature of being stable in a wide pH range (pH: 3-9). It does not need to be activated before use. It shows perfect material compatibility. It causes color change in medical devices that are not cleaned well and have biological residue on them. It causes biological residues to be fixed on the medical device and causes biofilm formation. It does not cause severe irritation on the eyes and respiratory tract mucosa, it does not require monitoring after exposure, it does not have an irritating odor. It is not carcinogenic. (Ortho phthal aldehyde) (ortho phthalaldehyde) (including OrthoPhthalaldehyde) (OrthoPhthalaldehyde) (including OrthoPhthalaldehyde) OPA, including skin, surface mucosa, clothing stains proteins by staining them gray. PPE such as gloves, eye and mouth protection, liquid-proof apron should be used during contact. In addition, medical devices should be thoroughly rinsed to prevent staining on the patient's mucosa or skin.

It is not recommended to use cystoscopes disinfected with OPA due to the risk of anaphylactic shock due to the residue of OPA (Ortho Phthalaldehyde) (Ortho Phthalaldehyde) (O-Phthalaldehyde) (Ortho-Phthalaldehyde) in patients with bladder cancer. Orthophthalaldehyde (Ortho Phthalaldehyde) (Ortho Phthalaldehyde) (orthophital aldehyde) (orthophthalaldehyde) (orthophtal aldehyde) (ortho phthalaldehyde) (orthophthal aldehyde) (ortho phthal aldehyde) (Phthalaldehyde) (O- OrthoFitalaldehyde) (OrthoPhthalaldehyde) (OPA) acts directly on nucleic acids, killing microorganisms by alkylation of cellular components. It is effective on all microorganisms and its effect on mycobacteria is better than glutaraldehyde. It is not sporicidal at normal pH. A concentration of 0.55% is recommended for use. Ortho Phthalaldehyde ) (OrthoPhthalaldehyde) OPA solution YDD time is 12 minutes in hand use and 5 minutes at 25 ° C in use with an automatic machine with temperature control.

Opened solution can be used for 14 days, unopened can has a shelf life of two years. As the concentration of water residues on medical devices will decrease during use, as with glutaraldehyde, it should be checked that it is at the effective concentration and test strips are used for this purpose. The minimum effective concentration is 0.3%. (Ortho Phthalaldehyde) (ortho phthalaldehyde) (orthophital aldehyde) (orthophthalaldehyde) (orthophital aldehyde) (ortho phthal aldehyde) (orthophthal aldehyde) (ortho phthal aldehyde) (Ortho Phthalaldehyde) (O- Phthalaldehyde) ) (OrthoPhthalaldehyde) Since OPA is ecotoxic, neutralization is required before being thrown into the sewage system as waste after use. Orthophthalaldehyde-based disinfectants are presented as ready-to-use solutions containing 0.55% active ingredient. Commercially available products can be used manually or in automatic disinfectors and washer disinfectors. Studies have shown that orthophthalaldehyde has the best microbiological effect of glutaraldehyde. Orthophthalaldehyde needs to be in contact for a long time to be effective against glutaraldehyde resistant mycobacteria. It does not generate harmful vapors, does not require activation and is stable over a wide pH range of 3 to 9. It has FDA approval. Exposure to orthophthalaldehyde vapor may cause irritation to the respiratory tract and eyes.

It is recommended to be used in well ventilated areas and well-sealed containers. Orthophthalaldehyde (ortho phthal aldehyde) (orthophthaldehyde) (orthophthalaldehyde) (orthophital aldehyde) (ortho phthal aldehyde) (orthophthal aldehyde) (ortho phthal aldehyde) advantages are that it is more effective and long-lasting (2 weeks) compared to gluteraldehyde. Orthophthalaldehyde concentration must always be controlled very accurately. Orthophthalaldehyde has some disadvantages. This disinfectant should be evaluated for a while in terms of its effects and properties. There is little information on safe contact levels and damage due to prolonged contact. Orthophthalaldehyde (ortho phthal aldehyde) (orthophthaldehyde) (orthophthalaldehyde) (orthophtal aldehyde) (orthophthal aldehyde) (orthophthal aldehyde) (ortho phthal aldehyde) causes proteins to coagulate biofilm). As a result of the reaction of amino and thiol groups, it may cause staining on the contact surface, tools, cotton fabrics and skin (Maillart reaction). Orthophthalaldehyde caused reactions similar to anaphylaxis after repeated use.

O-Phthalaldehyde or ortho-phthalaldehyde (Ortho Phthalaldehyde) (OrthoPhthalaldehyde) (ortho phthalaldehyde) (orthophital aldehyde) (orthophthalaldehyde) (orthophtal aldehyde) (ortho phthalaldehyde) (orthophthal aldehyde) (CHAhyOdehyde) ) It is a chemical compound with formula 2. Usually abbreviated OPA, the molecule is a dialdehyde consisting of two formyl (CHO) groups attached to neighboring carbon centers in a benzene ring. This pale yellow solid is a building block in the synthesis of heterocyclic compounds and a reagent in the analysis of amino acids. OPA dissolves in water solution at pH <11.5. Its solutions decompose upon UV illumination and exposure to air. (ortho phthal aldehyde) (orthophital aldehyde) (orthophthalaldehyde) (orthophital aldehyde) (orthophital aldehyde) (orthophthal aldehyde) (ortho phthal aldehyde) OPA (OrthoFitalaldehyde) (OrthoPhthalaldehyde) (Orthohythalaldehyde) (Phthahythalaldehyde) (Ortho-Phthalaldehyde) can be polymerized. In the polymer, one of the oxygen atoms bridges the other non-ring carbon of the same phthalaldehyde unit, while the other bridges bridge the non-ring carbon of another phthalaldehyde unit. Poly (phthalaldehyde) is used to create a photoresist.

(ortho phthal aldehyde) (orthophital aldehyde) (orthophthalaldehyde) (orthophital aldehyde) (ortho phital aldehyde) (orthophthal aldehyde) (ortho phthal aldehyde) Orthophthalaldehyde or (Ortho Phthalaldehyde) (Ortho Phthalaldehyde) (Ortho Phthalaldehydehyde) (Ortho Phthalaldehydehyde) (OrthoFitalaldehyde) (OrthoPhthalaldehyde) OPA is a dialdehyde used in the synthesis of heterocyclic compounds and a reagent in the analysis of amino acids. (Ortho Phthalaldehyde) (O- Phthalaldehyde) (ortho phthalaldehyde) (orthophtaldehyde) (orthophthalaldehyde) (orthophtaldehyde) (ortho phthalaldehyde) (orthophthal aldehyde) (ortho phthal aldehyde) (Ortho phthalhyhydehyde) It is widely used in hospitals as a medical disinfectant and as a surgical instrument sterilization solution for patient care. Orthophthalaldehyde can help disinfect a wide range of endoscopes and other heat sensitive critical semi-medical devices.
Ortho-Phthalaldehyde (Ortho Phthalaldehyde) (Ortho Phthalaldehyde) (O- Phthalaldehyde) (Ortho-Phthalaldehyde) (ortho phthalaldehyde) (orthophtaldehyde) (orthophthalaldehyde) (orthophital aldehyde) (ortho phthalaldehyde) (ortho phthalaldehyde) (ortho phthalaldehyde) (ortho-phthalaldehyde) ) (OrthoPhthalaldehyde) (OrthoPhthalaldehyde) (OPA) is an aromatic dialdehyde used as a high-end antimicrobial disinfectant for medical equipment sensitive to normal heat or steam sterilization processes, including endoscopes, cystoscopes, and some dental instruments.

For 40 years, glutaraldehyde, another dialdehyde, has been the primary choice for disinfecting heat sensitive medical devices; However, it has been reported to be a chemical sensitiser. Glutaraldehyde is known to have high affinity for biological amines, and its use as a tissue fixative benefits from this property. Therefore, glutaraldehyde and dialdehydes, a class of chemicals, can bind to natural proteins, thus changing their presentation to the immune system. Floraldehyde Reagent Solution (Ortho Phthalaldehyde) (Ortho Phthalaldehyde) (O- Phthalaldehyde) (Ortho-Phthalaldehyde) (ortho-phthalaldehyde) (ortho-phthalaldehyde) (ortho-phthalaldehyde) (ortho-phthalaldehyde) (ortho-phthalaldehyde) ) (orthophital aldehyde) (ortho phthalaldehyde) (orthophthal aldehyde) (ortho phthal aldehyde) (OrthoPhthalaldehyde) (OrthoPhthalaldehyde) Contains OPA (o-phthalaldehyde). Reagent that can be used as a protein or peptide assay reagent or as a pre- or post-column detection reagent for amino acid analysis (HPLC). (Ortho Phthalaldehyde) (O- Phthalaldehyde) (Ortho-Phthalaldehyde) (Ortho-phthalaldehyde) (ortho phthalaldehyde) (orthophtaldehyde) (orthophthalaldehyde) (orthophtaldehyde) (ortho phthalaldehyde) (ortho phthalaldehyde) (ortho phthalaldehyde) ) (OrthoPhthalaldehyde) OPA's reaction with proteins and peptides gives linear results in a wide range of concentrations. Fluoraldehyde Reagent Solution is supplied ready-to-use and enables rapid quantification of proteins or peptides in solution.

Ortho-phthalaldehyde (OPA) is a high-level disinfectant that received FDA clearance in October 1999. It contains at least 0.55% 1,2-benzenedicarboxaldehyde or OPA and has replaced glutaraldehyde as the most commonly used "aldehyde" at high levels. Level disinfection in the United States. OPA solution is a clear, pale blue liquid with a pH of 7.5. OPA's advantages, disadvantages, and features are listed in Table 301-2. In the chemical sterilant (disinfection) area, phthalaldehyde does not irritate the eyes and nasal passages compared to glutaraldehyde, but has excellent stability over a wide pH range (3-9), it does not require monitoring of exposure and has a barely perceptible odor. But phthaldialdehyde stains proteins, including unprotected skin, gray. Therefore, contaminated tools should be handled using gloves, eye protection, liquid resistant gowns when working with contaminated equipment and chemicals.

Pure ortho-phthalaldehyde is rarely encountered. When used as a disinfectant, it comes in the form of an aqueous solution with a concentration of about 0.55%. Commercial solutions contain additives that help stabilize their pH, such as citric acid and phosphates, preservatives, and colorants. Ortho-phthalaldehyde is primarily used as a high-efficiency chemical disinfectant for dental or medical instruments such as endoscopes. It is generally considered a safer alternative to glutaraldehyde. It is also used as a reagent for fluorometric analysis of primary amines and thiols in the laboratory. Phthalaldehyde (sometimes o-phthalaldehyde or ortho-phthalaldehyde, OPA) is the chemical compound with the formula C6H4 (CHO) 2. It is one of the three isomers of benzene dicarbaldehyde related to phthalic acid. This pale yellow solid is a building block in the synthesis of heterocyclic compounds and a reagent in the analysis of amino acids. OPA is soluble in water solution with pH <11.5. Their solutions degrade upon exposure to UV illumination and air.

O-Phthalaldehyde is used in a very sensitive fluorescent reagent to test amines or sulfhydryls in solution, especially in proteins, peptides and amino acids by capillary electrophoresis and chromatography. OPA reacts specifically with primary amines above the isoelectric point Pi in the presence of thiols. OPA also reacts with thiols in the presence of an amine such as n-propylamine or 2-aminoethanol. The method is spectrometric (fluorescent emission at 436-475 nm (max. 455 nm) with excitation at 330-390 nm (max. 340 nm)). O-Phthalaldehyde  is a chemical reagent that forms fluorescent conjugation products with primary amines. It is used for the detection of many biogenic amines, peptides and proteins in nanogram amounts in body fluids. O-Phthalaldehyde is FDA approved for use in test systems to detect blood urea nitrogen (BUN) for the diagnosis and treatment of certain kidney and metabolic diseases.

OPA is also a known disinfectant and has been approved for high level sterilization of heat sensitive medical instruments and is increasingly being used as a replacement for glutaraldehyde in the healthcare industry. OPA is also approved for use as an indoor antimicrobial pesticide; An intermediate for the synthesis of pharmaceuticals, drugs and other organic compounds. Nitrogen through the O-Phthaldialdehyde Assay (NOPA) is one of the methods used in winemaking to measure the yeast assimilable nitrogen (or YAN) needed by wine yeast to successfully complete fermentation. Ortho-phthalaldehyde: A possible alternative to glutaraldehyde for high-level disinfection. Ortho-phthalaldehyde (OPA) has been tested against a range of organisms, including glutaraldehyde-resistant mycobacteria, Bacillus subtilis spores, and coating-defective spores. Glutaraldehyde (glutaraldehyde) and peracetic acid (PAA) were tested for comparison. Both suspension and vehicle tests were conducted using various concentrations and exposure times. In suspension tests, all three biocides were very effective against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa (5 log reduction).

OPA and glutaraldehyde (PAA not tested) were also very effective against Staph. aureus and Ps. aeruginosa in carrier tests. OPA showed good activity against mycobacteria tested, including two glutaraldehyde resistant strains, but 0.5% w / v OPA was found to be non-sporicidal. However, limited activity was found at higher concentrations and pH values. Spores defective in the coating were more susceptible to OPA, suggesting that the coating may be responsible for this resistance. The findings of this study show that OPA is effective against glutaraldehyde-resistant mycobacteria and is a suitable alternative to glutaraldehyde for high-level disinfection.
This study was initiated to investigate the biocidal properties of OPA against a number of acid-fast, non-sporulating organisms, glutaraldehyde-sensitive and resistant mycobacteria and B. subtilis spores. A suspension test and a carrier test were used to evaluate the activity of biocides; This has been deemed necessary as high activity disinfectants have been shown in suspension tests to be not necessarily active on contaminated surfaces (Best et al., 1988). Spores treated with urea / dithiothreitol / sodium lauryl sulfate (UDS), pH 10 · 3 were also used.

Removal of protein from the spore coating has been shown to dramatically increase the sensitivity of B. subtilis to alkaline glutaraldehyde (Gorman et al. 1984) and the same effect was thought to occur with OPA. Comparisons with glutaraldehyde and PAA were made to evaluate OPA activity. Despite the high numbers of bacteria needed to measure large daily reductions in these experiments, all three biocides tested were found to be very effective against non-acid-fast spore-forming organisms. OPA, like the other two biocides, was still very effective at concentrations much lower than the recommended 0 · 5% (w / v) concentration for use and was equally effective against both Gram-negative and Gram-positive test bacteria. Due to the increase in the number of organisms tested, a direct comparison could not be made between the results of the suspension tests and the vehicle tests. However, both concentrations of OPA and glutaraldehyde were still effective with only 10 minutes of exposure to both test organisms and the recommended in-use concentrations of 0 5% (w / v) and 2 (5 log reduction) of OPA. and% (v / v) effective for glutaraldehyde, respectively