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Ortho phthalaldehyde is a white to pale yellow solid that is soluble in water, alcohols, and some organic solvents.
Ortho phthalaldehyde is primarily used as a disinfectant and sterilizing agent.
Ortho phthalaldehyde is a highly effective antimicrobial compound, particularly against bacteria, fungi, and viruses.
CAS Number: 643-79-8
Molecular Formula: C8H6O2
Molecular Weight: 134.13
EINECS Number: 211-402-2
Synonyms: Aldehyde, ortho-Phthalic, o Phthalaldehyde, o Phthaldialdehyde, Ortho phthalaldehyde, o-Phthaldialdehyde, ortho Phthalaldehyde, ortho Phthalic Aldehyde, orthOrtho phthalaldehyde, ortho-Phthalic Aldehyde, Orthophthaldialdehyde, Phtalaldehydes [French], Phthalaldehyde; Phthalic aldehyde, Phthalic dialdehyde, Phthalyldicarboxaldehyde, o-Phthaldialdehyde, 1,2-Benzenedicarboxaldehyde, [ChemIDplus] Cidex OPA, [Merck Index] OPA, 1,2-Benzenedialdehyde, 1,2-Diformylbenzene, 1,2-Phthalaldehyde, 2-Formylbenzaldehyde;, o-Benzenedicarbaldehyde, Benzenedicarboxaldehyde, OP 100S; OP 100SF, o-Phthalic aldehyde, Phthalic dicarboxaldehyde, Phtharal, [NTP] UN2923.
Ortho phthalaldehyde or orthOrtho phthalaldehyde is the chemical compound with the formula C6H4(CHO)2.
Ortho phthalaldehyde has a broad spectrum of activity and is often used in medical and laboratory settings for disinfection of medical instruments, endoscopes, and other equipment.
Ortho phthalaldehyde is also used in the pharmaceutical industry as a disinfectant for drug manufacturing facilities and in water treatment applications.
One of the notable features of Ortho phthalaldehyde is its rapid action and effectiveness even at low concentrations.
Ortho phthalaldehyde has been widely used as an alternative to glutaraldehyde, another commonly used disinfectant, due to its superior performance and reduced toxicity.
Ortho phthalaldehyde has been found to be less irritating to the skin and respiratory system compared to glutaraldehyde.
Ortho phthalaldehyde is a cyclic compound with a structure consisting of a central benzene ring with two aldehyde functional groups (-CHO) attached to adjacent carbon atoms.
Ortho phthalaldehydes chemical formula is C₈H₆O₂, and its molecular weight is 134.13 grams per mole.
Ortho phthalaldehyde has a melting point of around 64-66 °C (147-151 °F) and a boiling point of approximately 258-260 °C (496-500 °F).
Ortho phthalaldehyde is sparingly soluble in nonpolar solvents but dissolves readily in polar solvents such as water, alcohols, and ethers.
There are some researches show, pH7.5 contains the sterilizing agent of Ortho phthalaldehyde 0.5%, and its sterilizing power, sterilization speed, stability and toxicity all are better than glutaraldehyde, can kill mycobacterium in the 5min, the bacterium number reduces by 5 logarithmic value, and Ortho phthalaldehyde is very stable, tasteless in pH3～9 scopes, non-stimulated to human nose, eye mucosa, and need not activate before using, various materials are had good consistency, have tangible microbiocidal activity.
Ortho phthalaldehyde is the chemical compound with the formula C6H4(CHO)2.
Ortho phthalaldehyde is one of three isomers of benzene dicarbaldehyde, related to phthalic acid.
This pale yellow solid is a building block in the synthesis of heterocyclic compounds and a reagent in the analysis of amino acids.
Ortho phthalaldehyde dissolves in water solution at pH < 11.5.
Ortho phthalaldehydes solutions degrade upon UV illumination and exposure to air.
Ortho phthalaldehyde was first described in 1887 when it was prepared from α,α,α’,α’-tetrachloro-o-xylene.
A more modern synthesis is similar: the hydrolysis of the related Ortho phthalaldehyde using potassium oxalate, followed by purification by steam distillation.
Ortho phthalaldehyde, also known as OPA, belongs to the class of organic compounds known as benzoyl derivatives.
These are Ortho phthalaldehydes containing an acyl moiety of benzoic acid with the formula (C6H5CO-).
Ortho phthalaldehyde has been identified in human blood as reported by (PMID: 31557052 ).
Ortho phthalaldehyde is not a naturally occurring metabolite and is only found in those individuals exposed to this compound or its derivatives.
Technically Ortho phthalaldehyde is part of the human exposome.
Ortho phthalaldehyde can be defined as the collection of all the exposures of an individual in a lifetime and how those exposures relate to health.
An individual's Ortho phthalaldehyde begins before birth and includes insults from environmental and occupational sources.
Ortho phthalaldehyde, reacts with all primary amine-containing analytes to yield fluorescent isoindole derivatives.
Ortho phthalaldehyde, provides an accurate measure of both composition and absolute protein-peptide content.
Ortho phthalaldehyde, ıdeal for work with recombinant proteins and synthetic peptides.
Ortho phthalaldehyde, can be used for fluorescent protein or peptide assay
Ortho phthalaldehyde, pre-column derivatization mixtures can be injected into LC without any processing
Fluoraldehyde Reagent Solution contains OPA (Ortho phthalaldehyde), which reacts with primary amines of amino acids, peptide and proteins to enable fluorescent detection and quantitation.
Ortho phthalaldehyde that can be used as a protein or peptide assay reagent or as a pre- or post-column detection reagent for amino acid analysis (HPLC).
Reaction of Ortho phthalaldehyde with proteins and peptides yields linear results over a wide range of concentrations.
The Ortho phthalaldehyde is supplied ready to use and enables fast quantitation of proteins or peptides in solution.
The reactivity of Ortho phthalaldehyde is complicated by the fact that in water it forms both a mono- and dihydrate, C6H4(CHO)(CH(OH)2) and C6H4(CH(OH))2O, respectively.
Ortho phthalaldehydes reactions with nucleophiles often involves the reaction of both carbonyl groups.
Ortho phthalaldehyde(OPA)-amine reaction and OPA-amine-thiol reaction have been developed to effectively modify native peptides and proteins under the physiological conditions.
Ortho phthalaldehyde is mainly used as a high-level disinfectant (a low-temperature chemical method) for heat-sensitive medical and dental equipment such as endoscopes and thermometers; in recent years, it has gained popularity as a safe and better alternative to glutaraldehyde.
Ortho phthalaldehyde, pale pale yellow solid is a building block in the synthesis of heterocyclic compounds and a reagent in the analysis of amino acids.
Ortho phthalaldehyde is a building block in the synthesis of heterocyclic compounds and a reagent in the analysis of amino acids.
Often abbreviated OPA, the molecule is a dialdehyde, consisting of two formyl (CHO) groups attached to adjacent carbon centres on a benzene ring.
Ortho phthalaldehyde is a dialdehyde consisting of two formyl groups attached to adjacent carbon centres on a benzene ring.
Ortho phthalaldehyde is a compound that reacts with primary amines to produce a product that emits a highly fluorescent blue colour.
Ortho phthalaldehyde is a pale yellow crystalline solid.
Ortho phthalaldehyde, also known as OPDA or 1,2-benzenedicarboxaldehyde, is an organic compound with the chemical formula C₈H₆O₂.
Melting point: 55-58 °C(lit.)
Boiling point: 83-84 °C (0.7501 mmHg)
Density: 1.13
vapor pressure: 0.56Pa at 25℃
refractive index: 1.4500 (estimate)
Flash point: >230 °F
storage temp.: 2-8°C
solubility: The solubility of Ortho phthalaldehyde is 3g/100 mL diisopropyl ether, 5g/100mL deionized water, 20g/100mL chloroform, or 20g/100mL acetone at 20°C.
form: powder
color: yellow
PH: 7 (53g/l, H2O, 20℃)
Water Solubility: soluble
Sensitive: Air Sensitive
Merck: 14,7368
BRN: 878317
Exposure limits ACGIH: SL .025 mg/100 cm2; Ceiling 0.1 ppb (Skin)
Stability: Stable. Air sensitive. Incompatible with strong oxidizing agents, strong bases.
LogP: 0.99 at 30℃
Ortho phthalaldehyde disrupts the integrity of microbial cell walls and membranes, inhibits enzymatic activity, and interferes with essential cellular processes, ultimately leading to microbial death.
Ortho phthalaldehyde is compatible with various materials commonly used in medical and laboratory settings, such as stainless steel, glass, rubber, and plastics like polyethylene and polypropylene.
However, Ortho phthalaldehyde may cause discoloration or damage to certain materials, such as polycarbonate, acrylics, and some types of elastomers.
Ortho phthalaldehyde is recommended to perform compatibility testing on sensitive materials before prolonged exposure to Ortho phthalaldehyde.
Ortho phthalaldehyde is widely used in analytical chemistry as a reagent for the determination and quantification of various compounds.
Ortho phthalaldehyde is commonly employed as a derivatizing agent for amino acids and proteins in high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) analyses.
Ortho phthalaldehyde reacts with primary amines to form highly fluorescent derivatives, allowing for sensitive detection and quantification of amino acids and other amine-containing compounds.
Ortho phthalaldehyde has a strong odor, often described as pungent or unpleasant.
Proper ventilation is recommended when working with Ortho phthalaldehyde.
Ortho phthalaldehyde is sensitive to light and may undergo photochemical degradation.
Therefore, it is advisable to store Ortho phthalaldehyde in amber-colored containers and protect it from direct light exposure.
The pH of the solution can affect the stability and reactivity of Ortho phthalaldehyde.
Generally, a slightly acidic pH (around 4-5) is optimal for its derivatization reactions.
Ortho phthalaldehyde exhibits rapid and potent antimicrobial activity, making it highly effective against a wide range of microorganisms, including bacteria, fungi, and viruses.
Ortho phthalaldehyde is known for its ability to kill bacterial spores, which are highly resistant to many disinfectants.
Compared to other disinfectants like glutaraldehyde, Ortho phthalaldehyde is generally less toxic, less irritating to the skin and respiratory system, and less likely to cause allergic reactions.
Ortho phthalaldehyde has a shorter contact time requirement for effective disinfection, which can improve workflow efficiency in healthcare and laboratory settings.
Ortho phthalaldehyde is a dialdehyde in which two formyl groups are attached to adjacent carbon centres on a benzene ring.
Ortho phthalaldehyde, in the presence of 2-mercaptoethanol, reacts with primary amines to form highly fluorescent products.
Phthalaldehyde , primary amine and a sulfhydryl.
Ortho phthalaldehyde can be manufactured by oxidation of phthalan by nitrogen monoxide in acetonitrile with N-hydroxyphthalimide as the catalyst to yield 80% to 90%.
Ortho phthalaldehyde disinfectant is composed of the following raw materials by weight percent: 0.5-1% of Ortho phthalaldehyde, 0.5-5% of ASK solution (which is obtained by adding alkylene aliphatic sulfonic anhydride in an alkaline solution to react), 5-20% of short chain alcohol, 0.1-1% of complexing agent, 0.2-2% of pH buffer agent, and the balance of deionized water. By adopting the compound form of the Ortho phthalaldehyde disinfectant and the alkylene aliphatic sulfonic anhydride, good synergic sterilization effect and no foam are realized, and the spraying and immersion disinfection can be satisfied.
Ortho phthalaldehyde is relatively stable under normal storage conditions.
Ortho phthalaldehyde should be stored in a cool, dry place away from direct sunlight.
Ortho phthalaldehyde is important to keep Ortho phthalaldehyde containers tightly sealed to prevent exposure to air, moisture, and contaminants.
Over time, Ortho phthalaldehyde may undergo gradual oxidation, leading to a yellow discoloration.
However, this does not significantly affect its disinfection properties.
Ortho phthalaldehyde exerts its antimicrobial activity by interacting with cellular components of microorganisms, primarily proteins and enzymes.
Uses:
Ortho phthalaldehyde is widely used as a disinfectant and sterilizing agent due to its potent antimicrobial properties.
Ortho phthalaldehyde is effective against a broad spectrum of microorganisms, including bacteria, fungi, and viruses.
Ortho phthalaldehyde is commonly employed in healthcare settings for disinfection of medical instruments, such as endoscopes, surgical equipment, and dialysis equipment.
Ortho phthalaldehyde is also utilized in pharmaceutical manufacturing facilities to disinfect equipment and surfaces involved in drug production.
Ortho phthalaldehyde is used in water treatment applications to control microbial growth and eliminate bacteria and other microorganisms in water systems.
Ortho phthalaldehyde can be employed in various settings, including municipal water treatment plants, swimming pools, spas, and water storage tanks.
Ortho phthalaldehyde is utilized in analytical chemistry as a derivatizing agent for the quantification and detection of amino acids, peptides, and other amine-containing compounds.
Ortho phthalaldehyde reacts with primary amines to form highly fluorescent derivatives, enabling sensitive detection and analysis in techniques such as high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS).
Ortho phthalaldehyde is an active area of research and development.
Research efforts focus on areas such as wastewater treatment, development of new disinfection strategies, and enhancement of its stability and antimicrobial capabilities.
Ortho phthalaldehyde is sometimes used in dental care as a disinfectant.
It can be utilized for disinfection of dental instruments and equipment to help prevent cross-contamination and maintain proper infection control.
Ortho phthalaldehyde finds applications in veterinary and animal care settings.
Ortho phthalaldehyde can be used to disinfect medical instruments and equipment used in veterinary clinics, animal hospitals, and research facilities.
Ortho phthalaldehyde has been explored for its potential use in food processing and preservation.
Ortho phthalaldehyde may have applications in disinfecting equipment used in food production, such as food processing machinery and utensils, to ensure food safety and prevent microbial contamination.
Ortho phthalaldehyde is used in pharmaceutical research and development for its antimicrobial properties.
Ortho phthalaldehyde may be incorporated into formulations or studied for its potential as an active ingredient in antimicrobial drugs or disinfectant solutions.
Ortho phthalaldehyde has been investigated for its effectiveness in controlling microbial growth and preventing disease outbreaks in aquaculture settings.
Ortho phthalaldehyde may be utilized in fish farms and aquaculture facilities to disinfect equipment, tanks, and water systems.
Ortho phthalaldehyde is commonly used in research laboratories for disinfection purposes.
It can be employed to sterilize laboratory equipment, glassware, and surfaces to maintain a sterile and contamination-free environment.
Ortho phthalaldehyde is used in hemodialysis units for the disinfection of dialyzers, which are essential components of the hemodialysis process.
Ortho phthalaldehyde helps in preventing cross-contamination and maintaining a sterile environment during dialysis treatments.
Ortho phthalaldehyde is employed in the biotechnology and biopharmaceutical industries.
Ortho phthalaldehyde can be used for the disinfection of equipment and surfaces in bioprocessing facilities, laboratories, and cleanrooms to ensure the integrity and safety of biopharmaceutical products.
Ortho phthalaldehyde is sometimes utilized in environmental monitoring and testing.
It can be employed to disinfect sampling equipment used in environmental studies, water quality testing, and microbial analysis to prevent contamination and maintain the accuracy of results.
Ortho phthalaldehyde can be used in heating, ventilation, and air conditioning (HVAC) systems for the disinfection of cooling coils, condensate pans, and air handling units.
Ortho phthalaldehyde helps in controlling microbial growth and preventing the spread of contaminants through the air.
Ortho phthalaldehyde is used in sterile processing departments of hospitals and healthcare facilities.
Ortho phthalaldehyde can be applied for the disinfection and sterilization of medical devices, surgical instruments, and other equipment used in surgical procedures and patient care.
Ortho phthalaldehyde continues to be explored in research and development for new applications and improvements in existing uses.
Scientists are investigating its potential in various fields, such as antimicrobial coatings, antimicrobial textiles, and innovative disinfection methods.
Ortho phthalaldehyde can be used in the preservation of biological samples in laboratories and research facilities.
Ortho phthalaldehyde helps to inhibit microbial growth and maintain the integrity of the samples for extended periods.
Ortho phthalaldehyde can be widely used for precolumn derivatization of amino acids in Ortho phthalaldehyde separation or Capillary electrophoresis.
For flow cytometric measurements of protein thiol groups.
Precolumn derivatization reagent for primary amines and amino acids.
The fluorescent derivative can be detected by reverse-phase Ortho phthalaldehyde.
The reaction requires Ortho phthalaldehyde, primary amine and a sulfhydryl.
Ortho phthalaldehyde is primarily used as a disinfectant and sterilizing agent in various industries, including healthcare, pharmaceuticals, and water treatment.
Ortho phthalaldehyde is commonly employed for disinfecting medical equipment, such as endoscopes, surgical instruments, and dialysis equipment.
Ortho phthalaldehyde is also utilized in the manufacturing of pharmaceutical products as a disinfectant for facilities and equipment involved in drug production.
In water treatment, Ortho phthalaldehyde can be used to control microbial growth and eliminate bacteria and other microorganisms in water systems.
Ortho phthalaldehyde is a high-level chemical disinfectant that is commonly used for disinfection of dental and medical instruments as an alternative to glutaraldehyde, which is a known skin and respiratory sensitizer.
Ortho phthalaldehyde is used as a disinfectant and as a tanning agent in leather industry.
Ortho phthalaldehyde is useful for the sterilization of endoscopic instruments, thermometers, rubber and plastic equipment which cannot be sterilized by heating system.
Ortho phthalaldehyde is also used as an intermediate in synthesis of pharmaceuticals, medicines, and other organic compounds.
Ortho phthalaldehyde(OPA) is used for precolumn derivatization of amino acids for HPLC separation and for flow cytometric measurements of protein thiol groups.
Ortho phthalaldehyde used for fluorometric determination of histamine, histidine and other amino acids.
Also Ortho phthalaldehyde used for cholesterol assay in the picomole range.
Ortho phthalaldehyde has been used in the preparation of O-phthaldialdehyde reagent for analysing gentamycin content.
Ortho phthalaldehyde in the preparation of reagent for determining the degree of hydrolysis of milk proteins.
Ortho phthalaldehyde in the measurement of free amino acids of milk samples by O-phthaldialdehyde/N-acetyl-L-cysteine (OPA/NAC) assay.
Ortho phthalaldehyde is used for the quick visualization of histamine, characterized by the appearance of a yellow stain.
Ortho phthalaldehyde is used in the following products: biocides (e.g. disinfectants, pest control products).
Ortho phthalaldehyde is used in the following areas: health services.
Other release to the environment of Ortho phthalaldehyde is likely to occur from: indoor use as processing aid and indoor use in close systems with minimal release (e.g. cooling liquids in refrigerators, oil-based electric heaters).
Ortho phthalaldehyde used as a disinfectant and in the fluorometric determination of primary amines and thiols; [Merck Index] Used to sterilize medical and dental equipment, as an enzyme inhibitor, indicator, chemical intermediate, diagnostic agent, tanning agent for leather, in water treatment, pulp and paper manufacturing, oil field water flooding, hair colorings, wood treatment, and antifouling paints.
Ortho phthalaldehyde has been used; in the preparation of O-phthaldialdehyde reagent for analysing gentamycin content, in the preparation of reagent for determining the degree of hydrolysis of milk proteins, in the measurement of free amino acids of milk samples by o-phthaldialdehyde/N-acetyl-L-cysteine (OPA/NAC) assay, in the derivatization of putrescine samples.
Ortho phthalaldehyde is mainly used as a high-level disinfectant (a low-temperature chemical method) for heat-sensitive medical and dental equipment such as endoscopes and thermometers; in recent years, Ortho phthalaldehyde has gained popularity as a safe and better alternative to glutaraldehyde.
Safety Profile:
Ortho phthalaldehyde is considered to have a relatively low environmental impact compared to some other disinfectants.
It is biodegradable and does not persist in the environment for extended periods.
However, like any chemical, Ortho phthalaldehyde should be handled and disposed of responsibly to minimize any potential environmental impact.
The primary routes of human exposure to Ortho phthalaldehyde are by inhalation and through the skin, which may occur through accidental or occupational exposures.
Along with its increasing popularity as a chemical sterilizer, Ortho phthalaldehyde has many applications in analytical methods and in diagnostic kits.
Ortho phthalaldehyde is also used as an intermediate in the synthesis of pharmaceuticals and as a reagent in the tanning industry, hair colorings, wood treatment, and antifouling paints.
Ortho phthalaldehyde was approved for use as an indoor antimicrobial pesticide in 1997; however, it is no longer registered with the United States Environmental Protection Agency (USEPA) for this use.
Ortho phthalaldehyde can be a respiratory and skin irritant, and prolonged or repeated exposure may cause sensitization.
It is recommended to handle Ortho phthalaldehyde in a well-ventilated area and use appropriate personal protective equipment (PPE), such as gloves, goggles, and a lab coat, to minimize exposure.
Adhering to recommended usage concentrations and following safety guidelines provided by manufacturers or regulatory bodies is essential to ensure safe handling and minimize potential risks.
