PEG 1500

Synonyms:

25322-68-3; PEG 1500; Polyethylene oxide; Polyglycol; Polyoxy ethylene; Carbowax®; Polyglycol; Polyethylene glycol 200, 300, 400, 600,1000,1450, 3350, 4000, 6000, 8000 and 20000;ETHYLENE GLYCOL;1,2-ethanediol;Ethane-1,2-diol;107-21-1;glycol;monoethylene glycol;1,2-Dihydroxyethane;2-hydroxyethanol; Glycol alcohol; Ethylene alcohol; polyethylene glycol ;Macrogol; Fridex; Tescol; Ethylene dihydrate; Norkool; Macrogol 400 BPC ;Dowtherm SR 1; Carbowax 400;CCRIS 3744;Carbowax 1000;Dowtherm 1500;1,2-ethylene glycol;Ethylene glycol polymer;HSDB 5012;NCI-C00920;HOCH2CH2OH;Union Carbide XL 54 Type I De-icing Fluid;PEG 3350;EINECS 203-473-3;M.e.g.;Ethylene glycol homopolymer;Polyethylene glycol 1000;Polyethylene Glycol 1500;EPA Pesticide Chemical Code 042203;1,2-Ethanediol homopolymer;FC72KVT52F;AI3 03050; PEG; CHEBI:30742; LYCAIKOWRPUZTN-UHFFFAOYSA-N; PEG 1500;1, 2-Ethanediol;DuPont Zonyl FSO Fluorinated Surfactants;alpha-Hydro-omega-hydroxypoly(oxyethylene); DSSTox_CID_597; H(OCH2CH2)nOH ;DSSTox_RID_75680; DSSTox_GSID_20597; alpha-Hydro-omega-hydroxypoly(oxy-1,2-ethanediyl); Glycol, polyethylene; Miralax; Polyethylene oxide; CAS-107-21-1;Polyethylene Glycols ;Polyethylene glycol3350; Athylenglykol;  Aquaffin; Badimol; Carbowax; Modopeg;Nosilen;Nycoline;ehtylene glycol;ethylen glycol;ethylene-glycol;etylene glycol;Carbowax Sentry;Pluracol E;Polyaethylenglykol;Aquacide III;Ilexan E;Bradsyn PEG;ethylene alcohol;Merpol OJ;Polyaethylenglykole;MEG 100;Alkox SR;Oxide Wax AN; PEG200?300?400?600?800?1000?1500?2000?3000?1500?6000 ?8000,Polyglycol, Polyethylene oxide, Polyoxy ethylene,PEG; Carbowax®; Polyglycol; Polyethylene glycol 200, 300, 400, 600,1000,1450, 3350, 4000, 6000, 8000 and 20000;ETHYLENE GLYCOL;1,2-ethanediol;Ethane-1,2-diol;107-21-1;glycol;monoethylene glycol;1,2-Dihydroxyethane;2-hydroxyethanol;Glycol alcohol;Ethylene alcohol;polyethylene glycol;Macrogol;Fridex;Tescol;Ethylene dihydrate;Norkool;Macrogol 400 BPC;Dowtherm SR 1;Carbowax 400;CCRIS 3744;Carbowax 1000;Dowtherm 1500;1,2-ethylene glycol;Ethylene glycol polymer;HSDB 5012;NCI-C00920;HOCH2CH2OH;Union Carbide XL 54 Type I De-icing Fluid;PEG 3350;EINECS 203-473-3;M.e.g.;Ethylene glycol homopolymer;Polyethylene glycol 1000;Polyethylene Glycol 1500;EPA Pesticide Chemical Code 042203;1,2-Ethanediol homopolymer;FC72KVT52F;AI3-03050;PEG;CHEBI:30742;LYCAIKOWRPUZTN-UHFFFAOYSA-N;PEG 1500;1, 2-Ethanediol;DuPont Zonyl FSO Fluorinated Surfactants;alpha-Hydro-omega-hydroxypoly(oxyethylene);DSSTox_CID_597;H(OCH2CH2)nOH;DSSTox_RID_75680;DSSTox_GSID_20597;alpha-Hydro-omega-hydroxypoly(oxy-1,2 ethanediyl); Glycol, polyethylene;Miralax;Polyethylene oxide;CAS-107-21-1;Polyethylene Glycols;Polyethylene glycol 350;Athylenglykol;Aquaffin; Badimol; Carbowax; Modopeg ;Nosilen ;Nycoline ;ehtylene glycol; ethylen glycol; ethylene-glycol; etylene glycol ;Carbowax Sentry; Pluracol E; Polyaethylenglykol; Aquacide III; IlexanE;Bradsyn PEG;ethylene alcohol;Merpol OJ;Polyaethylenglykole;MEG 100;Alkox SR;Oxide WaxPOLİETİLEN GLİKOL,POLYETİLENGLİCOL, POLİETHİLENE  GLYİCOL, POLİETHELENE ; pOLYethylen glikol 1500; Polyethylene glycol  1500; Polyethylene glycol1500;  Polyethylene glycol-1500; Polyethylene Glycol  1500;  Polyethyleneglycol  1500; Polyethylene glycol/1500; Polietilen Glikol; Polietilen Glikol 1500; Polietilen Glikol1500; Polietilen glikol 1500; Polietilen Glikol 1500; POLİETİLEN GLİKOL 1500; POLİETİLEN GLİKOL1500; POLİETİLEN GLİKOL/1500; POLİETİLEN GLİKOL-1500;  POLİETİLEN GLİKOL 1500; POLIETILEN GLIKOL 1500; POLIETILEN GLIKOL1500; POLIETILEN GLIKOL/1500; POLIETILEN GLIKOL-1500; pEG 1500; PEG; PEG 1500; peg1500;  peg-1500;  peg/1500; peg1500

PEG 1500
Properties of PEG 1500
Related Categories    Essential Chemicals, Poly(ethylene glycol) (PEG) and PEG Solutions, Research Essentials Less...
form      solution
mol wt of PEG 1500      Mr ~1500
packaging  of PEG 1500     pkg of 10 × 4 mL
mfr. no.      Roche
shipped in      wet ice
storage temp.      2-8°C
SMILES string      C(CO)O
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Description of PEG 1500
General description of PEG 1500
Poly(ethylene glycol) ( PEG 1500) is a non-ionic hydrophilic polymer and is available in different molecular weights. It helps in the purification and crystal growth of proteins and nucleic acids. PEG and dextran together result in aqueous polymer two phase system, which is required for the purification of biological materials. PEG also interacts with cell membrane, thereby allowing cell fusion.[4][5]

Application of PEG 1500
Polyethylene Glycol 1500 (PEG 1500) has been used to mediate cell fusion.[1][2][3]

Physical form of PEG 1500
Solution, filtered through 0.2 μm pore size membrane, 50% PEG 1500 (w/v) in 75 mM Hepes (pH 8.0), bottled under nitrogen, ready to use

Other Notes of PEG 1500
For life science research only. Not for use in diagnostic procedures.

Product name  : Polyglycol PEG 1500

Grade of PEG 1500               : Extra pure

Synonym  of PEG 1500        : Polyglycol, Polyethylene oxide, Polyoxy ethylene, PEG 1500

Formula   of PEG 1500         : HO(C₂H₄O)nH

Description of PEG 1500
Cas no of PEG 1500 : 25322-68-3                    
EC no. of PEG 1500 : 500-038-2 
        
Product Description of PEG 1500
Application field of PEG 1500:
Pharmacology and Cosmetics production (as base for creams, toothpastes and lipsticks)
Detergent & Household goods production (as soap bars glue, soluble agent in detergent pastes, fixing agent for odors in soaps and detergents, as additive in general cleaners, polishers, air fresheners, automatic dishwashing detergents)
Production of textile supporting substances (component of dispergators and protective solutions)
Rubber goods production (non adhesive agent for forms treatment)
Metal works industry (agent for cleaning and polishing pastes, lubricating & cooling liquids).

Polyethylene glycol PEG 1500

Polyethylene glycol (PEG; /ˌpɒliˈɛθəlˌiːn ˈɡlaɪˌkɒl, -ˌkɔːl/) is a polyether compound derived from petroleum with many applications, from industrial manufacturing to medicine. PEG 1500 is also known as polyethylene oxide (PEO) or polyoxyethylene (POE), depending on its molecular weight. The structure of PEG 1500 is commonly expressed as H−(O−CH2−CH2)n−OH.[3]

Uses of PEG 1500
Medical uses
Main articles: Macrogol and PEGylation
PEG is the basis of a number of laxatives.[4] Whole bowel irrigation with polyethylene glycol and added electrolytes is used for bowel preparation before surgery or colonoscopy.
PEG 1500 is also used as an excipient in many pharmaceutical products.
When attached to various protein medications, polyethylene glycol allows a slowed clearance of the carried protein from the blood.[5]
The possibility that PEG could be used to fuse axons is being explored by researchers studying peripheral nerve and spinal cord injury.[4]
An example of PEG hydrogels (see "Biological uses" section) in a therapeutic has been theorized by Ma et al. They propose using the hydrogel to address periodontitis (gum disease) by encapsulating stem cells in the gel that promote healing in the gums.[6] The gel and encapsulated stem cells was to be injected to the site of disease and crosslinked to create the microenvironment required for the stem cells to function.
A PEGylated lipid is used as an excipient in both the Moderna and Pfizer–BioNTech vaccines for SARS-CoV-2. Both RNA vaccines consist of Messenger RNA, or mRNA, encased in a bubble of oily molecules called lipids. Proprietary lipid technology is used for each. In both vaccines, the bubbles are coated with a stabilizing molecule of polyethylene glycol.[medical citation needed] As of December 2020 there is some concern that PEG could trigger allergic reaction,[7] and in fact allergic reactions are the driver for both the UK and Canadian regulators to issue an advisory, noting that: two individuals "individuals in the U.K... were treated and have recovered" from anaphylactic shock.[8][9] As of 18 December, the US CDC stated that in their jurisdiction six cases of "severe allergic reaction" had been recorded from more than 250,000 vaccinations, and of those six only one person had a "history of vaccination reactions".[10]
Chemical uses

The remains of the 16th century carrack Mary Rose undergoing conservation treatment with PEG in the 1980s

Terra cotta warrior, showing traces of original color
Because PEG is a hydrophilic molecule, it has been used to passivate microscope glass slides for avoiding non-specific sticking of proteins in single-molecule fluorescence studies.[11]
Polyethylene glycol has a low toxicity and is used in a variety of products.[12] The polymer is used as a lubricating coating for various surfaces in aqueous and non-aqueous environments.[13]
Since PEG is a flexible, water-soluble polymer, it can be used to create very high osmotic pressures (on the order of tens of atmospheres). It also is unlikely to have specific interactions with biological chemicals. These properties make PEG one of the most useful molecules for applying osmotic pressure in biochemistry and biomembranes experiments, in particular when using the osmotic stress technique.
Polyethylene glycol is also commonly used as a polar stationary phase for gas chromatography, as well as a heat transfer fluid in electronic testers.
PEG has also been used to preserve objects that have been salvaged from underwater, as was the case with the warship Vasa in Stockholm,[14] and similar cases. It replaces water in wooden objects, making the wood dimensionally stable and preventing warping or shrinking of the wood when it dries.[4] In addition, PEG is used when working with green wood as a stabilizer, and to prevent shrinkage.[15]
PEG has been used to preserve the painted colors on Terracotta Warriors unearthed at a UNESCO World Heritage site in China.[16] These painted artifacts were created during the Qin Shi Huang (first emperor of China) era. Within 15 seconds of the terra-cotta pieces being unearthed during excavations, the lacquer beneath the paint begins to curl after being exposed to the dry Xi'an air. The paint would subsequently flake off in about four minutes. The German Bavarian State Conservation Office developed a PEG preservative that when immediately applied to unearthed artifacts has aided in preserving the colors painted on the pieces of clay soldiers.[17]
PEG is often used (as an internal calibration compound) in mass spectrometry experiments, with its characteristic fragmentation pattern allowing accurate and reproducible tuning.
PEG 1500 derivatives, such as narrow range ethoxylates, are used as surfactants.
PEG 1500 has been used as the hydrophilic block of amphiphilic block copolymers used to create some polymersomes.[18]
PEG 1500 has also been used as a propellent on the UGM-133M Trident II Missile, in service with the United States Air Force.[19]
Biological uses of PEG 1500
PEG can be modified and crosslinked into a hydrogel and used to mimic the extracellular matrix (ECM) environment for cell encapsulation and studies.[20][21]
An example study was done using PEG-Diacrylate hydrogels to recreate vascular environments with the encapsulation of endothelial cells and macrophages. This model furthered vascular disease modeling and isolated macrophage phenotype's effect on blood vessels.[22]
PEG is commonly used as a crowding agent in in vitro assays to mimic highly crowded cellular conditions.[11]
PEG is commonly used as a precipitant for plasmid DNA isolation and protein crystallization. X-ray diffraction of protein crystals can reveal the atomic structure of the proteins.
PEG is used to fuse two different types of cells, most often B-cells and myelomas in order to create hybridomas. César Milstein and Georges J. F. Köhler originated this technique, which they used for antibody production, winning a Nobel Prize in Physiology or Medicine in 1984.[4]
Polymer segments derived from PEG polyols impart flexibility to polyurethanes for applications such as elastomeric fibers (spandex) and foam cushions.
In microbiology, PEG precipitation is used to concentrate viruses. PEG is also used to induce complete fusion (mixing of both inner and outer leaflets) in liposomes reconstituted in vitro.
Gene therapy vectors (such as viruses) can be PEG-coated to shield them from inactivation by the immune system and to de-target them from organs where they may build up and have a toxic effect.[23] The size of the PEG polymer has been shown to be important, with larger polymers achieving the best immune protection.
PEG is a component of stable nucleic acid lipid particles (SNALPs) used to package siRNA for use in vivo.[24][25]
In blood banking, PEG is used as a potentiator to enhance detection of antigens and antibodies.[4][26]
When working with phenol in a laboratory situation, PEG 300 can be used on phenol skin burns to deactivate any residual phenol (some references are required).
In biophysics, polyethylene glycols are the molecules of choice for the functioning ion channels diameter studies, because in aqueous solutions they have a spherical shape and can block ion channel conductance.[27][28]
Commercial uses
PEG is the basis of many skin creams (as cetomacrogol) and personal lubricants (frequently combined with glycerin).
PEG is used in a number of toothpastes[4] as a dispersant. In this application, it binds water and helps keep xanthan gum uniformly distributed throughout the toothpaste.
PEG is also under investigation for use in body armor, and in tattoos to monitor diabetes.[29][30]
In low-molecular-weight formulations (e.g. PEG 400), it is used in Hewlett-Packard designjet printers as an ink solvent and lubricant for the print heads.
PEG is also used as an anti-foaming agent in food and drinks[31] – its INS number is 1521[32] or E1521 in the EU.[33]
Industrial uses
A nitrate ester-plasticized polyethylene glycol (NEPE-75) is used in Trident II submarine-launched ballistic missile solid rocket fuel.[34]
Dimethyl ethers of PEG are the key ingredient of Selexol, a solvent used by coal-burning, integrated gasification combined cycle (IGCC) power plants to remove carbon dioxide and hydrogen sulfide from the gas waste stream.
PEG has been used as the gate insulator in an electric double-layer transistor to induce superconductivity in an insulator.[35]
PEG is also used as a polymer host for solid polymer electrolytes. Although not yet in commercial production, many groups around the globe are engaged in research on solid polymer electrolytes involving PEG, with the aim of improving their properties, and in permitting their use in batteries, electro-chromic display systems, and other products in the future.
PEG is injected into industrial processes to reduce foaming in separation equipment.
PEG is used as a binder in the preparation of technical ceramics.[36]
Recreational uses
PEG is used to extend the size and durability of very large soap bubbles.
PEG is the main ingredient in many personal lubricants. (Not to be confused with propylene glycol.)
Health effects
PEG is considered biologically inert and safe by the FDA. However, a growing body of evidence shows the existence of a detectable level of anti-PEG antibodies in approximately 72% of the population, never treated with PEGylated drugs, based on plasma samples from 1990–1999.[37] The FDA has been asked to investigate the possible effects of PEG in laxatives for children. Since 1999, the FDA has received over 1,000 incident reports from parents reporting serious or life threatening side effects after their children were given one or more doses of PEG as an osmotic laxative.[38] Miralax has not been tested on children. PEG is not recommended to those under 18.

Due to its ubiquity in a multitude of products and the large percentage of the population with antibodies to PEG, hypersensitive reactions to PEG are an increasing concern.[39][40] Allergy to PEG is usually discovered after a person has been diagnosed with an allergy to an increasing number of seemingly unrelated products, including processed foods, cosmetics, drugs, and other substances that contain PEG or were manufactured with PEG.[39]

When PEG is chemically attached to therapeutic molecules (such as protein drugs or nanoparticles), it can sometimes be antigenic (a molecule which stimulates an immune response), stimulating an anti-PEG antibody response in some patients. This effect has only been shown for a few of the many available PEGylated therapeutics, but it has significant effects on clinical outcomes of affected patients.[41] Other than these few instances where patients have anti-PEG immune responses, it is generally considered to be a safe component of drug formulations.[medical citation needed]

Available forms and nomenclature
PEG, PEO, and POE refer to an oligomer or polymer of ethylene oxide. The three names are chemically synonymous, but historically PEG is preferred in the biomedical field, whereas PEO is more prevalent in the field of polymer chemistry. Because different applications require different polymer chain lengths, PEG has tended to refer to oligomers and polymers with a molecular mass below 20,000 g/mol, PEO to polymers with a molecular mass above 20,000 g/mol, and POE to a polymer of any molecular mass.[42] PEGs are prepared by polymerization of ethylene oxide and are commercially available over a wide range of molecular weights from 300 g/mol to 10,000,000 g/mol.[43]

PEG and PEO are liquids or low-melting solids, depending on their molecular weights. While PEG and PEO with different molecular weights find use in different applications, and have different physical properties (e.g. viscosity) due to chain length effects, their chemical properties are nearly identical. Different forms of PEG are also available, depending on the initiator used for the polymerization process – the most common initiator is a monofunctional methyl ether PEG, or methoxypoly(ethylene glycol), abbreviated mPEG. Lower-molecular-weight PEGs are also available as purer oligomers, referred to as monodisperse, uniform, or discrete. Very high-purity PEG has recently been shown to be crystalline, allowing determination of a crystal structure by x-ray crystallography.[43] Since purification and separation of pure oligomers is difficult, the price for this type of quality is often 10–1000 fold that of polydisperse PEG.

PEGs are also available with different geometries.

Branched PEGs have three to ten PEG chains emanating from a central core group.
Star PEGs have 10 to 100 PEG chains emanating from a central core group.
Comb PEGs have multiple PEG chains normally grafted onto a polymer backbone.
The numbers that are often included in the names of PEGs indicate their average molecular weights (e.g. a PEG with n = 9 would have an average molecular weight of approximately 400 daltons, and would be labeled PEG 400.) Most PEGs include molecules with a distribution of molecular weights (i.e. they are polydisperse). The size distribution can be characterized statistically by its weight average molecular weight (Mw) and its number average molecular weight (Mn), the ratio of which is called the polydispersity index (ĐM). Mw and Mn can be measured by mass spectrometry.

PEGylation is the act of covalently coupling a PEG structure to another larger molecule, for example, a therapeutic protein, which is then referred to as a PEGylated protein. PEGylated interferon alfa-2a or −2b are commonly used injectable treatments for hepatitis C infection.

PEG is soluble in water, methanol, ethanol, acetonitrile, benzene, and dichloromethane, and is insoluble in diethyl ether and hexane. It is coupled to hydrophobic molecules to produce non-ionic surfactants.[44]

PEGs potentially contain toxic impurities, such as ethylene oxide and 1,4-dioxane.[45] Ethylene Glycol and its ethers are nephrotoxic if applied to damaged skin.[46]

Polyethylene oxide (PEO, Mw 4 kDa) nanometric crystallites (4 nm)
PEG and related polymers (PEG phospholipid constructs) are often sonicated when used in biomedical applications. However, as reported by Murali et al., PEG is very sensitive to sonolytic degradation and PEG degradation products can be toxic to mammalian cells. It is, thus, imperative to assess potential PEG degradation to ensure that the final material does not contain undocumented contaminants that can introduce artifacts into experimental results.[47]

PEGs and methoxypolyethylene glycols are manufactured by Dow Chemical under the trade name Carbowax for industrial use, and Carbowax Sentry for food and pharmaceutical use. They vary in consistency from liquid to solid, depending on the molecular weight, as indicated by a number following the name. They are used commercially in numerous applications, including foods, in cosmetics, in pharmaceutics, in biomedicine, as dispersing agents, as solvents, in ointments, in suppository bases, as tablet excipients, and as laxatives. Some specific groups are lauromacrogols, nonoxynols, octoxynols, and poloxamers.

Macrogol, MiraLax, GoLytely, Colace used as a laxative, is a form of polyethylene glycol. The name may be followed by a number which represents the average molecular weight (e.g. macrogol 3350, macrogol 4000 or macrogol 6000).

Production

Polyethylene glycol 400, pharmaceutical quality

Polyethylene glycol 4000, pharmaceutical quality
The production of polyethylene glycol was first reported in 1859. Both A. V. Lourenço and Charles Adolphe Wurtz independently isolated products that were polyethylene glycols.[48] Polyethylene glycol is produced by the interaction of ethylene oxide with water, ethylene glycol, or ethylene glycol oligomers.[49] The reaction is catalyzed by acidic or basic catalysts. Ethylene glycol and its oligomers are preferable as a starting material instead of water, because they allow the creation of polymers with a low polydispersity (narrow molecular weight distribution). Polymer chain length depends on the ratio of reactants.

HOCH2CH2OH + n(CH2CH2O) → HO(CH2CH2O)n+1H
Depending on the catalyst type, the mechanism of polymerization can be cationic or anionic. The anionic mechanism is preferable because it allows one to obtain PEG with a low polydispersity. Polymerization of ethylene oxide is an exothermic process. Overheating or contaminating ethylene oxide with catalysts such as alkalis or metal oxides can lead to runaway polymerization, which can end in an explosion after a few hours.

Polyethylene oxide, or high-molecular weight polyethylene glycol, is synthesized by suspension polymerization. It is necessary to hold the growing polymer chain in solution in the course of the polycondensation process. The reaction is catalyzed by magnesium-, aluminium-, or calcium-organoelement compounds. To prevent coagulation of polymer chains from solution, chelating additives such as dimethylglyoxime are used.

Alkaline catalysts such as sodium hydroxide (NaOH), potassium hydroxide (KOH), or sodium carbonate (Na2CO3) are used to prepare low-molecular-weight polyethylene glycol.