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TWEEN 80=POLYSORBATE 80
CAS number: 9005-65-6
EC number: 500-019-9
Chemical formula: C64H124O26
Molar mass: 1310 g/mol
IUPAC name: Polyoxyethylene (20) sorbitan monooleate
DESCRIPTION:
TWEEN 80 has been used in drug vehicle solution for specific injections in mice and rats.
TWEEN 80 has been used for the determination of esterase activity in bacterial cells.
Non-ionic detergent used for selective protein extraction and isolation of nuclei from mammalian cell lines.
Many detergent varieties are available from several suppliers molecular and biochemical procedures such as protein purification, DNA/RNA isolation, staining and microscopy, electrophoresis, immunoassays, and more.
Tween 80 also known as polysorbate 80, is a nonionic detergent commonly used in cell lysis for protein and nucleic acid extraction.
Tween 80 is also added to immunoassay buffers such as PBS or TBS.
At room temperature, Tween 80 appears as a clear, viscous amber-colored liquid.
Tween 80 has a molar mass of 1,310 g/mol and is soluble in water and alcohol.
TWEEN 80 is a nonionic surfactant and emulsifier often used in foods and cosmetics.
TWEEN 80 is a viscous, water-soluble yellow liquid.
Tween 80 is an ethoxylated sorbitan ester based on a natural fatty acid (oleic acid).
This ethoxylate is highly effective at forming oil in water emulsions, particularly when used in combination with its non-ethoxylated precursor, Span 80.
Tween 80 (polysorbat 80) is recommended by the European Pharmacopeia as surface-active additive for solubilisation of heavily wettable reagents (1 g/l).
Tween 80, (=Polyoxyethylene Sorbitan Monooleate), also known as Polyoxyethylene Sorbitan Monooleate, is a nonionic emulsifier and surfactant and used in cosmetics and foods.
Tween-80 is a highly-purified detergent stabilized as a 10% solution (w/v) and packaged under nitrogen in glass ampules or non-leaching HDPE bottles, ensuring it's stability and eliminating the accumulation of peroxides and degradation products.
Tween 80 is a 100% bio-based non-ionic surfactant used as versatile emulsifier, dispersing agent, functional carrier fluid, and solubiliser for applications ranging from decorative and industrial coatings to adhesives and liquid colourants.
Tween 80 is derived from polyethoxylated sorbitan and oleic acid.
The hydrophilic groups in Tween 80 are polyethers also known as polyoxyethylene groups, which are polymers of ethylene oxide.
Tween 80 is found in pharmaceuticals and food preparation.
Tween 80 is often used in cosmetics to solubilize essential oils into water-based products.
Tween 80 is used as an emulsifier in combination with a variety of other hydrophobic emulsifiers to cover a wide range of oil in water, and water in oil emulsion systems.
Individually, Tween 80 is an excellent solubilizer of essential oils, wetting agent, viscosity modifier, stabilizer and dispersing agent.
Tween 80, 100ml is a nonionic surfactant solution used as a supplement in various culture media in a laboratory setting.
The name Polysorbate 80 is synonymous for Tween 80.
Tween 80, 100ml is a nonionic surfactant derived from sorbitan esters.
Tween 80 is used as an emulsifier and dispersing agent when included in various culture media formulas and serves to emulsify and disperse hydrophobic medium components.
Tween 80 will also act as a surfactant for samples from surface swabs, for food samples, cosmetics which have high fat content or hydrophobic content.
Tween 80 may be included as a component of a dehydrated culture medium i.e 7375 D/E Neutralizing Agar, or it may be added as a supplement to a medium base without Tween.
Tween 80, a substance formulated by the reaction of sorbitan fatty acid ester (a nonionic surfactant) with ethylene oxide, is used in many foreign countries, including the U.S. and the EU, where Tween 80 acts as an emulsifier, a solubilizer in many foods, including bread, cake mix, salad dressing, shortening oil and chocolate 18.
Tween 80 is a hydrophilic nonionic surfactant.
Tween 80 is utilized as a surfactant in soaps and cosmetics and also as a lubricant in eye drops.
In food or pharmaceutical products, Tween 80 can act as an emulsifier.
Tween 80 is an excipient that is used to stabilize aqueous formulations of medications for parenteral administration or vaccinations.
A solubilizing agent acts as a surfactant and increases the solubility of one agent in another.
A substance that would not normally dissolve in a particular solution is able to dissolve with the use of a solubilizing agent.
Tween 80 is also known as an emulsifier, which helps ingredients mix together and prevent separation, and water-containing small amounts of salts, and is included in several vaccines licensed in the USA.
TWEEN 80 is a polyethylene sorbitol ester, also know as Polysorbate 80, PEG (80) sorbitan monooleate, polyoxyethylenesorbitan monooleate.
TWEEN 80 is widely used in biochemical applications including: solubilizing proteins, isolating nuclei from cells in culture, growing of tubercule bacilli, and emulsifying and dispersing substances in medicinal and food products.
TWEEN 80 has little or no activity as an anti-bacterial agent except TWEEN 80 has been shown to have an adverse effect on the antibacterial effect of methyl paraben and related compounds.
Polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid.
They may reduce the activity of many preservatives.
CAS number: 9005-65-6
EC number: 500-019-9
Chemical formula: C64H124O26
Molar mass: 1310 g/mol
IUPAC name: Polyoxyethylene (20) sorbitan monooleate
Features of Tween-80 Detergent Solution:
• Tween 80—a popular nonionic detergent for use in ELISA, Western blotting and other immunoassay PBS or TBS wash buffers
• Accurate—precise 10% detergent solution in ultrapure water
• Easy-to-use—solution is simple to dispense and dilute for use
• Exceptionally pure—less than 1.0µeq/mL peroxides and carbonyls
• Stable—packaged under inert nitrogen gas in glass ampules or HDPE bottles
Specifications for Tween 80 Detergent Solution:
• Visual: Slightly hazy to cloudy, colorless solution, free of particulates; may form layers.
• Concentration: 10.0±1.0%
• Oxidants: ≤1.0µeq/mL
• Carbonyls: ≤1.0µeq/mL
• Suspended Solids: Residue present must not exceed Residue Reference.
Pharmacodynamics:
Polysorbates are widely used to protect biological drug products from protein unfolding, aggregation, and precipitation during both shipping and handling.
The polysorbates are amphipathic, nonionic surfactants made of fatty acid esters of polyoxyethylene sorbitan.
Polysorbate 80 is one of the most common surfactants currently used in the formulation of protein-based biopharmaceuticals
Mechanism of action:
Polysorbate 80 is one of the primary components of protein formulations.
Polysorbate 80 inhibits interfacial damage of the protein molecule that undergoes mechanical stress during shipping and handling.
Polysorbate 80 also affects the formulation photostability.
Exposure to light of polysorbate 80 aqueous solution results in peroxide generation, which in turn may lead to oxidation of the susceptible amino acid residues in the protein molecule.
One study on rats found that Polysorbate 80 increased apical to basolateral membrane permeability of digoxin in Caco-2 cells suggesting that Ps 80 is an in vitro inhibitor of P-glycoprotein.
It concluded that PS 80 may influence in vivo absorption of P-gp substrates, and this could be translated to human applications.
Metabolism:
Polysorbates undergo degradation by auto-oxidation, producing reactive peroxides, in addition to hydrolysis.
Exposure to light of polysorbate 80 aqueous solution leads autoxidation of the alkyl polyoxyethylene chain leading to the production of hydroperoxide derivatives.
The peroxides cause oxidative damage to the protein molecule that makes up the formulation.
The residual peroxides and the rate of peroxide generation as a result of light exposure are found to vary for polysorbate 80 of different grades/from different sources.
CAS number: 9005-65-6
EC number: 500-019-9
Chemical formula: C64H124O26
Molar mass: 1310 g/mol
IUPAC name: Polyoxyethylene (20) sorbitan monooleate
Chemical description of TWEEN 80:
Ethoxylated sorbitan ester
Functions of TWEEN 80:
Emulsifier
Applications of TWEEN 80:
Bitumen & construction
Chemicals manufacture
Emulsification
Nonwovens
Chemistry of TWEEN 80:
Polysorbate 80 is derived from polyethoxylated sorbitan and oleic acid.
The hydrophilic groups in this compound are polyethers also known as polyoxyethylene groups, which are polymers of ethylene oxide.
In the nomenclature of polysorbates, the numeric designation following polysorbate refers to the lipophilic group, in this case, the oleic acid (see polysorbate for more detail).
The full chemical names for TWEEN 80 are:
Polyoxyethylene (20) sorbitan monooleate
(x)-sorbitan mono-9-octadecenoate poly(oxy-1,2-ethanediyl)
The critical micelle concentration of polysorbate 80 in pure water is reported as 0.012 mM.[3]
Other names:
E number: E433
Brand names:
Alkest TW 80
Scattics
Canarcel
Poegasorb 80
Kolliphor PS 80 - Kolliphor is a registered trademark of BASF
Montanox 80 – Montanox is a registered trademark of Seppic
Tween 80 – Tween is a registered trademark of Croda Americas, Inc.[4]
Uses of TWEEN 80:
Food:
Polysorbate 80 is used as an emulsifier in foods.
For example, in ice cream, polysorbate is added up to 0.5% (v/v) concentration to make the ice cream smoother and easier to handle, as well as increasing its resistance to melting.
Adding this substance prevents milk proteins from completely coating the fat droplets.
This allows them to join in chains and nets, which hold air in the mixture, and provide a firmer texture that holds its shape as the ice cream melts.
Health and beauty:
Polysorbate 80 is also used as a surfactant in soaps and cosmetics (including eyedrops), or a solubilizer, such as in a mouthwash.
The cosmetic grade of polysorbate 80 may have more impurities than the food grade.
Medical:
Polysorbate 80 is an excipient that is used to stabilize aqueous formulations of medications for parenteral administration, and used as an emulsifier in the making of the antiarrhythmic amiodarone.
TWEEN 80 is also used as an excipient in some European and Canadian influenza vaccines.
Influenza vaccines contain 2.5 μg of polysorbate 80 per dose.
TWEEN 80 is found in many vaccines used in the United States, including the Janssen COVID-19 vaccine.
TWEEN 80 is used in the culture of Mycobacterium tuberculosis in Middlebrook 7H9 broth.
TWEEN 80 is also used as an emulsifier in the estrogen-regulating drug Estrasorb.
Polysorbate 80 is also used in granulation for stabilization of drugs and excipients when IPA binding.
Laboratory:
Some mycobacteria contain a type of lipase (enzyme that breaks up lipid molecules); when these species are added to a mixture of polysorbate 80 and phenol red, they cause the solution to change color, so this is used as a test to identify the phenotype of a strain or isolate.
On RODAC agar plates used in microbiological control, polysorbate 80 counteracts disinfectants often found on sampled surfaces, thereby allowing the microbes found on these surfaces to grow.
CAS number: 9005-65-6
EC number: 500-019-9
Chemical formula: C64H124O26
Molar mass: 1310 g/mol
IUPAC name: Polyoxyethylene (20) sorbitan monooleate
Physicochemical Information of TWEEN 80:
Boiling point: >100 °C (1013 hPa)
Density: 1.07 g/cm3 (25 °C)
Flash point: >113 °C
pH value: 5 - 7 (50 g/l, H₂O, 20 °C)
Vapor pressure: <1.33 hPa (20 °C)
Viscosity kinematic: 300 - 500 mm2/s (25 °C)
Saponification value: 45 - 55
Hydroxyl value: 65 - 80
Identity (IR): passes test
Description:non-ionic
Form: viscous liquid
mol wt: micellar avg mol wt: 79,000
average mol wt: 1310
composition:
Oleic acid, ≥58.0% (balance primarily linoleic, palmitic, and stearic acids)
aggregation number:60
CMC: 0.012 mM (20-25°C)
transition temp: cloud point 65 °C
density: 1.064 g/cm3
HLB: 15
Solubility in water: 100 mL/L[2]
Solubility in other solvents: soluble in ethanol, cottonseed oil, corn oil, ethyl acetate, methanol, toluene
Detergent Class: Nonionic polyoxyethylene surfactant
Aggregation Number: 60
Micelle Molecular Weight: 76,000g
Critical Micelle Concentration (CMC): 0.012 mM (0.0016%, w/v)
Dialyzable: No
SAFETY INFORMATION ABOUT TWEEN 80:
First aid measures:
Description of first aid measures
• General information: No special measures required.
• After inhalation: Supply fresh air; consult doctor in case of complaints.
• After skin contact:
Immediately rinse with water.
If skin irritation continues, consult a doctor.
• After eye contact:
Rinse opened eye for several minutes under running water.
Seek medical treatment.
• After swallowing:
Rinse out mouth.
If symptoms persist consult doctor.
Firefighting measures
Extinguishing media
• Suitable extinguishing agents: Water, CO2, foam, powder.
Special hazards arising from the substance or mixture
Formation of toxic gases is possible during heating or in case of fire.
Advice for firefighters
• Protective equipment: Wear self-contained respiratory protective device.
• Additional information
Collect contaminated fire fighting water separately.
It must not enter the sewage system.
Dispose of fire debris and contaminated fire fighting water in accordance with official regulations.
Contain escaping vapours with water.
Accidental release measures:
Personal precautions, protective equipment and emergency procedures
Do not inhale steams/aerosols.
Environmental precautions: Do not allow to enter sewers/ surface or ground water.
Methods and material for containment and cleaning up:
Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).
Clean up affected area
Handling and Storage:
• Information about storage conditions: Keep container sealed.
• Recommended storage temperature: Room Temperature
Store sealed bottle containing the product at 15-30°C.
If opened and not completely utilized, be certain that aseptic dispensing procedures are followed to maintain sterility of the supplement.
Protect from contamination by keeping container tightly closed.
Exposure controls/personal protection
• Control parameters
• Ingredients with limit values that require monitoring at the workplace: Not required.
• Additional information: The lists valid during the making were used as basis.
• Exposure controls:
• Individual protection measures, such as personal protective equipment
• General protective and hygienic measures:
Immediately remove all soiled and contaminated clothing
• Respiratory protection:
Use suitable respiratory protective device only when aerosol or mist is formed.
Filter ABEK
• Hand protection:
The glove material has to be impermeable and resistant to the product/ the substance/ the
preparation.
Selection of the glove material on consideration of the penetration times, rates of diffusion and the
degradation
• Material of gloves
The selection of the suitable gloves does not only depend on the material, but also on further marks of quality and varies from manufacturer to manufacturer.
• Penetration time of glove material
The exact break through time has to be found out by the manufacturer of the protective gloves and has to be observed.
• For the permanent contact gloves made of the following materials are suitable:
Nitrile rubber, NBR
Recommended thickness of the material: ≥ 0.11 mm
Value for the permeation: Level ≥ 480 min
• As protection from splashes gloves made of the following materials are suitable:
Nitrile rubber, NBR
Recommended thickness of the material: ≥ 0.11 mm
Value for the permeation: Level ≥ 480 min
• Eye/face protection: Safety glasses
• Body protection:
Protective work clothing
Protective clothing should be selected specifically for the working place, depending on concentration
and quantity of the hazourdous substances handled.
CAS number: 9005-65-6
EC number: 500-019-9
Chemical formula: C64H124O26
Molar mass: 1310 g/mol
IUPAC name: Polyoxyethylene (20) sorbitan monooleate
SYNONYMS OF TWEEN 80:
Montanox 80
Alkest TW 80
PS 80
POLYSORBATE 80
