PRODUCTS

BETA-MERCAPTOETHANOL

CAS NO:60-24-2
EC NO:200-464-6

Beta-Mercaptoethanol appears as a water-white liquid. May be toxic by ingestion, inhalation, or skin absorption.
Beta-Mercaptoethanol is a primary alcohol and an alkanethiol.
Beta-Mercaptoethanol has a role as a geroprotector.
A water-soluble thiol derived from hydrogen sulfide and ethanol.
Beta-Mercaptoethanol is used as a reducing agent for disulfide bonds and to protect sulfhydryl groups from oxidation.

Industry Uses of Beta-Mercaptoethanol:
-Corrosion inhibitors and anti-scaling agents
-Intermediates
-Process regulators
-Processing aids, specific to petroleum production

Consumer Uses of Beta-Mercaptoethanol:
-Oil and gas drilling
-Water treatment products

Industry Processing Sectors:
-All other basic organic chemical manufacturing
-All other chemical product and preparation manufacturing
-Oil and gas drilling, extraction, and support activities
-Plastic material and resin manufacturing

Beta-Mercaptoethanol is the chemical compound with the formula HOCH2CH2SH.
Beta-Mercaptoethanol, as it is commonly abbreviated, is used to reduce disulfide bonds and can act as a biological antioxidant by scavenging hydroxyl radicals (amongst others).
Beta-Mercaptoethanol is widely used because the hydroxyl group confers solubility in water and lowers the volatility. Due to its diminished vapor pressure, its odor, while unpleasant, is less objectionable than related thiols.

Production:
Beta-Mercaptoethanol is manufactured industrially by the reaction of ethylene oxide with hydrogen sulfide. Thiodiglycol and various zeolites catalyze the reaction.

Properties:
Chemical formula:   C2H6OS
Molar mass:   78.13 g·mol−1
Odor:   Disagreeable, distinctive
Density:   1.114 g/cm3
Melting point:   −100 °C (−148 °F; 173 K)
Boiling point:   157 °C; 314 °F; 430 K
log P:   -0.23
Vapor pressure:   0.76 hPa (at 20 °C) 4.67 hPa (at 40 °C)
Acidity (pKa):   9.643
Basicity (pKb):   4.354
Refractive index (nD):   1.4996

Beta-Mercaptoethanol is widely used for retarding oxidation of biological compounds in solution.
Beta-Mercaptoethanol is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.
Beta-Mercaptoethanol is a thiol compound, commonly used as a reducing agent in organic reactions.

Beta-Mercaptoethanol (also known as beta-mercaptoethanol or BME) is a potent reducing agent used in cell culture media to prevent toxic levels of oxygen radicals.
Beta-Mercaptoethanol is not stable in solution, so most protocols require daily supplementation.

Beta-Mercaptoethanol can act as an enzyme reactivator in systems necessitating reduction for activation, and has been commonly used to reduce disulfide bonds in order to separate protein subunits for use in electrophoresis.
Beta-Mercaptoethanol can also be used to form o-Phthalaldehyde-beta-mercaptoethanol (OPAME), a fluorogenic reagent shown to detect amino acids based on its reaction with primary amines. More recently OPAME has been used to distinguish between ssDNA/dsDNA and even single base mismatches in the hybridization of DNA.

Technical Information:
Physical State :Liquid
Solubility :Soluble in water (500 g/l), alcohol, ether, benzene, and other organic solvents.
Storage :Store at room temperature
Melting Point :<-100° C
Boiling Point :157.2° C (dec.)
Density :1.11 g/mL at 25° C (lit.)
Refractive Index :n20D 1.50 (lit.)
pK Values :pKa: 9.64 (Predicted)

Beta-mercaptoethanol (BME) is a reducing agent often used in biochemistry applications for protein denaturing.
Beta-mercaptoethanol (BME) is a small molecule.
Beta-mercaptoethanol (BME) is the major active constituent of silymarin, a standardized extract of the milk thistle seeds, containing a mixture of flavonolignans consisting of beta Mercaptoethanol, isobeta Mercaptoethanol, silicristin, silidianin, and others.

Beta-Mercaptoethanol is also known as 2-mercaptoethanol, thioglycol, etc., commonly abbreviated as ME、2-Me or β-ME.
Beta-Mercaptoethanol, which contains the functional groups of ethanediol (HOCH2CH2OH) and dithioglycol (HSCH2CH2SH), plays an important role in cell growth. On the one hand, it can reduce the sulfur compounds in the serum to glutathione, induce cell proliferation and delay cell senescence, while avoiding the damage of cells to peroxide. In addition, it promotes mitogen response and DNA synthesis, and increases the transformation effect of plant lectins (PHA) on lymphocytes.
Beta-Mercaptoethanol has been widely used in hybridoma technology.

Beta-Mercaptoethanol is used to reduce disulfide bonds and can act as a biological antioxidant by scavenging hydroxyl radicals.
Beta-Mercaptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis.
Beta-Mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state.

Beta-Mercaptoethanol is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Chemical Properties
Clear colorless liquid. 2-mercaptoethanol (bME) is a popular effective reducer, but extremely pungent.

Uses
Beta-Mercaptoethanol is used in the synthesis of nano-graphene for cellular imaging and drug delivery. Also used in the synthesis of multifunctional polymeric micelle used in specific targeting of tumor disruption.

Uses
Solubilizes proteins by reducing disulfide linkages2-Mercaptoethanol is used in the preparation of nano-graphene for cellular imaging and drug delivery as well as multifunctional polymeric micelle.
Beta-Mercaptoethanol acts as a reducing agent used in electrophoresis, amino acid detection, and distinguishing ssDNA/dsDNA.
Beta-Mercaptoethanol is also employed as a standard buffer.
Beta-Mercaptoethanol is also used in the preparation of PVC heat stabilizers and as a chain transfer agent in the manufacture of PVC. Further, it is used in some RNA isolation procedures to eliminate ribonuclease.
Beta-Mercaptoethanol is utilized as a corrosion inhibitor and ore floatation agent. In biochemistry, it is useful to study the activity of the immune system.

About Beta-Mercaptoethanol
Helpful information
Beta-Mercaptoethanol is registered under the REACH Regulation and is manufactured in and / or imported to the European Economic Area, at ≥ 1 000 to < 10 000 tonnes per annum.

Beta-Mercaptoethanol is used by professional workers (widespread uses), in formulation or re-packing, at industrial sites and in manufacturing.

Consumer Uses
ECHA has no public registered data indicating whether or in which chemical products the substance might be used. ECHA has no public registered data on the routes by which Beta-Mercaptoethanol is most likely to be released to the environment.

Article service life
ECHA has no public registered data on the routes by which Beta-Mercaptoethanol is most likely to be released to the environment. ECHA has no public registered data indicating whether or into which articles the substance might have been processed.

Widespread uses by professional workers
Beta-Mercaptoethanol is used in the following products: pH regulators and water treatment products and laboratory chemicals.
Beta-Mercaptoethanol is used in the following areas: health services and scientific research and development.
Other release to the environment of Beta-Mercaptoethanol is likely to occur from: indoor use (e.g. machine wash liquids/detergents, automotive care products, paints and coating or adhesives, fragrances and air fresheners).
Formulation or re-packing
ECHA has no public registered data indicating whether or in which chemical products the substance might be used. Release to the environment of Beta-Mercaptoethanol can occur from industrial use: formulation of mixtures.

Uses at industrial sites
Beta-Mercaptoethanol is used in the following products: pH regulators and water treatment products and metal surface treatment products.
Beta-Mercaptoethanol has an industrial use resulting in manufacture of another substance (use of intermediates).
Beta-Mercaptoethanol is used in the following areas: mining and formulation of mixtures and/or re-packaging.
Beta-Mercaptoethanol is used for the manufacture of: chemicals.
Release to the environment of Beta-Mercaptoethanol can occur from industrial use: as an intermediate step in further manufacturing of another substance (use of intermediates), in processing aids at industrial sites, as processing aid and as processing aid.
Manufacture
Release to the environment of Beta-Mercaptoethanol can occur from industrial use: manufacturing of the substance.

Formula: C2H6OS / HSCH2CH2OH
Molecular mass: 78.1
Boiling point: 157°C
Melting point: <-100°C
Relative density (water = 1): 1.1
Solubility in water: miscible
Vapour pressure, kPa at 20°C: 0.13
Relative vapour density (air = 1): 2.7 (calculated)
Relative density of the vapour/air-mixture at 20°C (air = 1): 1.002
Flash point: 74°C o.c.
Auto-ignition temperature: 295°C
Explosive limits, vol% in air: 2.3-18
Octanol/water partition coefficient as log Pow: -0.3 (estimated)

IUPAC names:
2-Hydroxy-1-ethanethiol
2-Mercaptoethanol
2-mercaptoethanol
2-mercaptoethanol
2-sulfanylethan-1-ol
2-Sulfanylethanol
2-sulfanylethanol
Ethanol, 2-mercapto-
Ethanol, 2-mercapto-
Mercaptoethanol
Thioglycol

SYNONYMS:
200-464-6 [EINECS]
2-Hydroxyethylmercaptan
2-Mercaptoethanol [Wiki]
2-Sulfanylethanol [ACD/IUPAC Name]
2-Sulfanylethanol [German] [ACD/IUPAC Name]
2-Sulfanyléthanol [French] [ACD/IUPAC Name]
2-sulfhydryl-ethanol
60-24-2 [RN]
Ethanol, 2-mercapto- [ACD/Index Name]
mercaptoethanol [Wiki]
mercaptoethyl alcohol
MFCD00004890 [MDL number]
Monothioethylene glycol
Monothioethyleneglycol
thioethylene glycol
β-Hydroxyethanethiol
β-Hydroxyethylmercaptan
β-mercaptoethanol
β-Mercaptoethanol
&β;-mercaptoethanol
(2S,3S)-1,4-dimercaptobutane-2,3-diol
1,1,2,2-tetradeuterio-1-deuteriooxy-2-deuteriosulfanylethane
155-04-4 [RN]
155613-89-1 [RN]
1-Ethanol-2-thiol
1-Hydroxy-2-mercaptoethane
1-Mercapto-2-hydroxyethane
2 Mercaptoethanol
2,3-DIHYDROXY-1,4-DITHIOBUTANE
203645-37-8 [RN]
284474-53-9 [RN]
2-Hydroxy-1-Ethanethiol
2-Hydroxyethanethiol
2-Hydroxyethyl mercaptan
2-mercapto ethanol
2-Mercapto-1-ethanol
2-Mercaptobenzothiazole zinc salt
2-Mercaptoethanol-1,1,2,2-d4
2-Mercaptoethanol-d6
2-Mercaptoethyl alcohol
2-mercaptoethynol
2-Sulfanylethan-1-ol