L-HISTIDINE MONOHYDROCHLORIDE MONOHYDRATE

L-Histidine monohydrochloride monohydrate has been used as a component of broth to match the tuna meat amino acid composition.
L-Histidine Monohydrochloride Monohydrate has also been used for partial rescue of the IMPL2 (myo-inositol monophosphatase) phenotype during seed development of Arabidopsis.
L-Histidine Monohydrochloride Monohydrate is used as a precursor of histidine, it can be used to enhance the biosyntyhesis of lovastatin by cultured Aspergillus terreus

CAS Number: 5934‑29‑2 
EC Number: 611‑821‑4 (EINECS), also listed as 211‑438‑9 in some pharmacopeia 
MDL Number: MFCD00151027
Molecular Formula: C6H9N3O2.ClH / C6H10ClN3O2 
C₆H₉N₃O₂ · HCl · H₂O (Hill notation)
Molecular Weight: ~209.63 g/mol

SYNONYMS:
L‑histidine hydrochloride monohydrate, L‑histidine HCl hydrate, Histidine hydrochloride hydrate, L‑α‑amino‑β‑(4‑imidazolyl)propionic acid monohydrochloride hydrate, H‑His‑OH·HCl·H₂O, L‑histidine hydrochloride monohydrate, Histidine HCl hydrate, L-α-Amino-β-(4-imidazolyl)propionic acid monohydrochloride, L-Histidine hydrochloride, 645-35-2, 1007-42-7, Histidine hydrochloride, Histidine monohydrochloride, Histidine HCl, L-Histidine, monohydrochloride, L-Histidine hydrochloride (VAN), Histidine hydrochloride, L-, L-Histidine monohydrochloride, L-Histidine, hydrochloride (1:1), Histidine hydrochloride (VAN), (-)-Histidine monohydrochloride, EINECS 211-438-9, Histidine monohydrochloride, L-(+)-, NSC 257867, PHOTONYL, DTXSID3020700, Histidine monohydrochloride [NF], AI3-18473, 1D5Q932XM6, EINECS 213-754-2, DL-Histidine, hydrochloride, L-Histidine, monohydrochloride, hydrate, SUNACTYL LS 9610, OSMHYDRAN LS 8453, DTXCID70700, P.P.A.A., Histidine, hydrochloride (1:1), Histidine, monohydrochloride, EINECS 229-266-8, NSC 206275, NSC-257867, HISTIDINE HYDROCHLORIDE [WHO-DD], HISTIDINE, L-, MONOHYDROCHLORIDE, HISTIDINE MONOHYDROCHLORIDE ANHYDROUS, CCRIS 7814, L-HISTIDINE MONOHYDROCHLORIDE [FCC], Histidine hydrochloride, L, LHistidine, monohydrochloride, HISTIDINE HCL [INCI], LHistidine, hydrochloride (1:1), Histidine monohydrochloride, L(+), 645352: ()Histidine monohydrochloride, 7048024: LHistidine monohydrochloride, monohydrate, 211-438-9, 213-754-2, 7048-02-4, (S)-2-Amino-3-(1H-imidazol-4-yl)propanoic acid hydrochloride, H-His-OH.xHCl, l-Histidine.HCl, L-Hisidine monohydrocholoride, MFCD00064556, (2S)-2-amino-3-(1H-imidazol-5-yl)propanoic acid hydrochloride, NCGC00093950-01, (2S)-2-amino-3-(1H-imidazol-5-yl)propanoic acid;hydrochloride, (2S)-2-Amino-3-(1H-imidazol-4-yl)-propanoic acid hydrochloride, Histidine, hydrochloride, H-His-OH.HCl, H-His-OH.HCl.H2O, UNII-1D5Q932XM6, histidine, chloride, (2S)-2-amino-3-(1H-imidazol-4-yl)propanoic acid hydrochloride, SCHEMBL209483, SCHEMBL209484, (S)-2-Amino-3-(1H-imidazol-4-yl)propanoic acid HCl, orb2695117, CHEMBL1256578, Histidine, monohydrochloride, DL-, (-)-Histidine monohydrochloride; Histidine hydrochloride; Histidine monohydrochloride; L-Histidine hydrochloride, AAA64535, Tox21_111234, Tox21_500566, AKOS015898038, L-Histidine HCl (H-L-His-OH.HCl), CCG-221870, DS-6380, FD20036, LP00566, NCGC00261251-01, CAS-645-35-2, DB-029959, DB-308116, CS-0009863, EU-0100566, H0150, NS00079890, NS00079950, ST51037609, EN300-74584, H 8125, SR-01000075269, SR-01000075269-1, Q27252266, (S)-2-Amino-3-(1h-imidazol-5-yl)propanoic acid hydrochloride, S(+)-alpha-Amino-1H-imidazole-4-propanoic acid hydrochloride, 56272-24-3, 70605-39-9, L-α-Amino-4(or 5)-imidazolepropionic Acid Monohydrochloride ; L-Histidine Hydrochloride, L-α-Amino-β-(4-imidazolyl)propionic acid monohydrochloride,  (S)-α-amino-1H-imidazole-4-propanoic acid hydrochloride,  glyoxaline-5-alanine hydrochloride, His, 15N3, 98%), L-HIS HCL H2O, L-His.H2o.HCl, Histidine (H), HISTIDINE HCL, H-His-OH¤HCl¤H, L-HISTIDINEHCL, H-HIS-OH HCL H2O, L-His-OH•HCl•H2O, HISTIDINE HCL H2O

L-Histidine Monohydrochloride Monohydrate is freely soluble in water.
L-Histidine monohydrochloride monohydrate (L-Histidine hydrochloride hydrate, H-His-OH.HCl.H2O), a structural analogue of the essential amino acid L-histidine, is a reversible inhibitor of protein biosynthesis which evokes disparate responses from non-tumorigenic and tumorigenic cells in culture.

L-Histidine Monohydrochloride Monohydrate is the hydrochloride salt form of L-histidine, an essential amino acid that plays crucial roles in protein synthesis, enzyme catalysis, and neurotransmitter regulation.
L-Histidine Monohydrochloride Monohydrate aspect indicates that it contains one molecule of water per molecule of the compound.

In research, L-Histidine Monohydrochloride Monohydrate is often utilized in cell culture media to support cell growth and viability, as well as in protein purification and crystallization processes.
Its availability in a stable, crystalline form makes L-Histidine Monohydrochloride Monohydrate valuable for experimental reproducibility and accuracy in various biological assays and investigations.

L-Histidine Monohydrochloride Monohydrate is the hydrochloride salt hydrate of the essential amino acid L‑histidine.
L‑histidine is vital for protein synthesis, histamine production, enzyme function, and metal ion binding.

In biological systems, histidine’s imidazole side chain plays key roles in acid–base balance, catalytic sites in enzymes, and chelation of metal ions (e.g., in hemoglobin)
L-Histidine Monohydrochloride Monohydrate is a histidine precursor via histidine decarboxylase.

L-Histidine Monohydrochloride Monohydrate, also known as 2-Amino-3-(1H-imidazol-4-yl)propanoic acid, is an alpha-amino acid with an imidazole functional group.
L-Histidine Monohydrochloride Monohydrate is a hydrophilic essential amino acid that has a basic sidechain containing an imidazole group.

L-Histidine Monohydrochloride Monohydrate is biosynthesized from ATP, 5-phosphoribosyl-1-pyrophosphate (PRPP), and glutamine.
L-Histidine Monohydrochloride Monohydrate has been used to study cultures of the human T-lymphoblastic leukemia cell line MOLT-4 to study modulation of apoptosis.
Exogenous histidine has been shown to enhance the biosynthesis of lovastatin by cultured Aspergillus terreus.

USES and APPLICATIONS of L-HISTIDINE MONOHYDROCHLORIDE MONOHYDRATE:
L-Histidine Monohydrochloride Monohydrate is used in cell culture media formulations used in biomanufacturing.
L-Histidine Monohydrochloride Monohydrate has been used to study cultures of the human T-lymphoblastic leukemia cell line MOLT-4 to study modulation of apoptosis.

L-Histidine Monohydrochloride Monohydrate  is a compound commonly used in life science research, particularly in biochemistry and molecular biology.
L-Histidine monohydrochloride monohydrate has been used as a component of broth to match the tuna meat amino acid composition.

L-Histidine Monohydrochloride Monohydrate has also been used for partial rescue of the IMPL2 (myo-inositol monophosphatase) phenotype during seed development of Arabidopsis.
L-Histidine Monohydrochloride Monohydrate is used as a precursor of histidine, it can be used to enhance the biosyntyhesis of lovastatin by cultured Aspergillus terreus

L-Histidine Monohydrochloride Monohydrate is utilized as a single nitrogen source to probe swarming in Pseudomonas aeruginosa on agar
L-Histidine Monohydrochloride Monohydrate can be employed in laboraotry applications as a component to avoid the risk of contaminating bioproducts with adventitious viruses

L-Histidine Monohydrochloride Monohydrate is also used for laboratory study of cultures of the human T-lymphoblastic leukemia cell line, and modulation of apoptosis
Biochemical Reagent: L-Histidine Monohydrochloride Monohydrate is widely used in cell culture media, protein purification, pharmaceutical formulations, and biochemical research

Precursor to histamine: Through decarboxylation, part of histamine biosynthesis
Metabolic role: L-Histidine Monohydrochloride Monohydrate participates in synthesis of compounds like carnosine and anserine, known for antioxidant and anti-inflammatory roles

Feed additive: Evaluated by EFSA as a nutritional additive produced by fermentation for animal feeds
L-Histidine monohydrochloride monohydrate for biochemistry
L-Histidine monohydrochloride monohydrate is used as a precursor through histidine decarboxylase.

L-Histidine Monohydrochloride Monohydrate is also used to study the cultures of the human T-lymphoblastic leukemia cell line, modulation of apoptosis.
L-Histidine Monohydrochloride Monohydrate is also used to enhance the biosynthesis of Lovastatin by cultured aspergillus terreus.

L-Histidine Monohydrochloride Monohydrate is utilized as a single nitrogen source to probe swarming in Pseudomonas aeruginosa on agar.
L-Histidine Monohydrochloride Monohydrate is employed as a component to avoid the risk of contaminating bioproducts with adventitious viruses.
L-Histidine Monohydrochloride Monohydrate has been utilized as a single nitrogen source to probe swarming in Pseudomonas aeruginosa on agar.

NOTES of L-HISTIDINE MONOHYDROCHLORIDE MONOHYDRATE:
Store L-Histidine Monohydrochloride Monohydrate away from strong oxidizing agents.
Keep L-Histidine Monohydrochloride Monohydrate container tightly closed.
Store L-Histidine Monohydrochloride Monohydrate in cool, dry conditions in well sealed containers.

CHEMICAL PROPERTIES of L-HISTIDINE MONOHYDROCHLORIDE MONOHYDRATE:
L-Histidine Monohydrochloride Monohydrate is a white crystals or cryst. or granular powder.

L-Histidine Monohydrochloride Monohydrate is used in cell culture media formulations used in biomanufacturing.
L-Histidine Monohydrochloride Monohydrate has been used to study cultures of the human T-lymphoblastic leukemia cell line MOLT-4 to study modulation of apoptosis.

L-Histidine Monohydrochloride Monohydrate, non-animal, is used in cell culture media formulations used in biomanufacturing.
The use of non-animal-sourced L-Histidine Monohydrochloride Monohydrate and other components avoids the risk of contaminating bioproducts with adventitious viruses.

L-Histidine Monohydrochloride Monohydrate can be used as AhR activators to treat gluten-induced gastrointestinal diseases.
L-Histidine Monohydrochloride Monohydrate can also be used for hair coloring method.

Chemicals play an important role in the stabilization of a biologic drug during L-Histidine Monohydrochloride Monohydrate's manufacturing and formulation process – for instance, by preventing aggregation.

BIOCHEM/PHYSIOL ACTIONS of L-HISTIDINE MONOHYDROCHLORIDE MONOHYDRATE:
L-Histidine Monohydrochloride Monohydrate takes part in protein methylation.
L-Histidine Monohydrochloride Monohydrate is associated with the hemoglobin structure and function.
L-Histidine Monohydrochloride Monohydrate is present in antioxidative dipeptides.

L-Histidine Monohydrochloride Monohydrate is involved in one-carbon unit metabolism.
L-Histidine Monohydrochloride Monohydrate is biosynthesized from ATP, 5-phosphoribosyl-1-pyrophosphate (PRPP), and glutamine.

This essential amino acid can be degraded to glutamate by histidiase, urocanase and imidazolonepropionase; it is also the precursor of histamine by action of histidine decarboxylase.

PURIFICATION METHODS of L-HISTIDINE MONOHYDROCHLORIDE MONOHYDRATE:
Crystallise the monohydrochloride from aqueous EtOH or 60% aqueous EtOH (m 259odec).
Alternatively dissolve 10g in 50mL of H2O, decolourise with Norite, filter, evaporate it in a vacuum to a syrup, cool to room temperature, add 95% EtOH with stirring until slightly turbid, scratch the sides of the vessel until crystals form, then add slowly 40mL of EtOH and keep at 0o overnight, filter the solid off, wash it several times with EtOH and dry L-Histidine Monohydrochloride Monohydrate in a vacuum.

PHYSICAL and CHEMICAL PROPERTIES of L-HISTIDINE MONOHYDROCHLORIDE MONOHYDRATE:
Appearance: White or nearly white crystalline powder or crystals; odorless
Melting Point: Decomposes around 253–268 °C (typical ≈254 °C)
Density: Approx. 1.47 g/cm³ at 20 °C
Bulk density: ~770 kg/m³
Solubility: Freely soluble in water (~149–170 g/L at 20 °C; other sources note ~42 mg/mL / ~390 g/L)
Practically insoluble in ethanol; soluble in formic acid
pH of 10% aqueous solution: 3.5–4.5 at 20 °C
Specific Optical Rotation: +9.2° to +10.6° (C=11 in 6 M HCl at 20 °C)
Vapor Pressure: <1 hPa at 20 °C
CAS Number: 5934‑29‑2

EC Number: 611‑821‑4 (211‑438‑9)
Formula / M.W.: C₆H₉N₃O₂ · HCl · H₂O / ~209.63 g/mol
Appearance: White crystalline powder or crystals
Melting Point: ~254 °C (decomposition)
Density: ~1.47 g/cm³
Solubility: ~150–170 g/L water; insoluble in ethanol
pH (10%) solution: ~3.5–4.5
Optical Rotation: +9.2° to +10.6°
Main Use Areas: Research reagent, cell culture, feed additive

Biological Role: Amino acid for protein synthesis, histamine precursor
Purity & Impurities: ≥99%; low heavy metals and sulphate
Storage Conditions: 2–30 °C, dry, avoid moisture
CAS number: 5934-29-2
EC number: 211-438-9
Hill Formula: C₆H₁₀ClN₃O₂ * H₂O
Molar Mass: 209.63 g/mol
HS Code: 2933 29 90
Density: 1.49 g/cm3 (20 °C)
Melting Point: 256 - 268 °C (decomposition)
pH value: 3.5 - 4.5 (100 g/l, H₂O, 20 °C)
Vapor pressure: <1 hPa (20 °C)

Bulk density: 770 kg/m3
Solubility: 149.55 g/l
Synonyms: His
Hill Formula: C6H10ClN3O2 * H2O
HS Code: 2933 29 90
EC number: 211-438-9
Molar mass: 209.63 g/mol
CAS number: 5934-29-2
Solubility: 169.9 g/l (20 °C)
Melting point: 259 °C (decomposition)
Bulk density: 770 kg/m3

pH value: 3.5 - 4.5 (100 g/l, H2O, 20 °C)
CAS: 5934-29-2
IUPAC Name: (2S)-2-amino-3-(1H-imidazol-4-yl)propanoic acid hydrate hydrochloride
Molecular Formula: C6H12ClN3O3
InChI Key: CMXXUDSWGMGYLZ-XRIGFGBMSA-N
SMILES: O.Cl.NC@@HC(O)=O
Molecular Weight (g/mol): 209.63
Synonym: L-histidin hydrate hydrochloride
MDL Number: MFCD00151027
Form: Crystals or powder or crystalline powder or fused solid
Comment: Material Sourced in UK and US

Appearance (Color): White
Optical Rotation: 9.5 ± 1.0? (C=5 in 5N HCl)
Assay (Titration ex Chloride): ≥98.5 to ≤101.5%
Physical state: crystals
Color: colorless
Odor: weak characteristic odour
Melting point/freezing point: 256 - 268 °C at 1.013 hPa 
Initial boiling point and boiling range: Decomposes below the boiling point.

Flammability (solid, gas): No data available
Upper/lower flammability or explosive limits: No data available
Flash point: Not applicable
Autoignition temperature: No data available
Decomposition temperature: > 268 °C
pH: 4,38 - 4,48 at 149,55 g/l at 20 °C
Viscosity: Viscosity, kinematic: No data available, 
Viscosity, dynamic: No data available
Water solubility: 149,55 g/l at 20 °C - OECD Test Guideline 105
Partition coefficient: n-octanol/water: No data available

Vapor pressure: < 0,1 hPa at 20 °C - OECD Test Guideline 104
Density: 1,4723 g/cm3 at 20 °C - OECD Test Guideline 109
Relative density: No data available
Relative vapor density: No data available
Particle characteristics: No data available
Explosive properties: Not classified as explosive.
Oxidizing properties: none
Other safety information: Bulk density ca.770 kg/m3
Empirical Formula (Hill Notation): C6H9N3O2 · HCl · H2O
CAS Number: 5934-29-2

Molecular Weight: 209.63
Beilstein: 4168261
MDL number: MFCD00151027
UNSPSC Code: 12352209
PubChem Substance ID: 329757872
NACRES: NA.77
Molecular Weight: 191.61 g/mol
Hydrogen Bond Donor Count: 4
Hydrogen Bond Acceptor Count: 4
Rotatable Bond Count: 3

Exact Mass: 191.0461543 Da
Monoisotopic Mass: 191.0461543 Da
Topological Polar Surface Area: 92 Ų
Heavy Atom Count: 12
Formal Charge: 0
Complexity: 151
Isotope Atom Count: 0
Defined Atom Stereocenter Count: 1
Undefined Atom Stereocenter Count: 0
Defined Bond Stereocenter Count: 0
Undefined Bond Stereocenter Count: 0

Covalently-Bonded Unit Count: 2
Compound Is Canonicalized: Yes
Molecular Formula / Molecular Weight: C6H9N3O2·HCl·H2O = 209.64
Physical State (20 deg.C): Solid
Storage Temperature: Room Temperature (Recommended in a cool and dark place, <15°C)
Store Under Inert Gas: Store under inert gas
Condition to Avoid: Hygroscopic
CAS RN: 5934-29-2
Reaxys Registry Number: 4168261
PubChem Substance ID: 87570593
Merck Index (14): 4720
MDL Number: MFCD00151027

CAS Number: 5934-29-2
Molecular Formula: C6H9N3O2 • HCl • H2O
Molecular Weight: 209.63 g/mol
Beilstein Registry Number: 4168261
MDL Number: MFCD00151027
CAS: 5934-29-2
IUPAC Name: (2S)-2-amino-3-(1H-imidazol-4-yl)propanoic acid hydrate hydrochloride
Molecular Formula: C6H12ClN3O3
InChI Key: CMXXUDSWGMGYLZ-XRIGFGBMSA-N
SMILES: O.Cl.NC@@HC(O)=O
Molecular Weight (g/mol): 209.63

Synonym: L-histidin hydrate hydrochloride
MDL Number: MFCD00151027
Form: Powder or crystals or crystalline powder
Assay (special): Chloride: 16.6-17.1%
Impurity content: Sulfate: ≤0.02%
pH: 3.5-4.5
Assay from Supplier's CofA: ≥98.0%
Heavy Metals: ≤10 ppm
Loss on Drying: ≤0.2%
Appearance (Color): White to off-white
Identification (FTIR): Conforms

Impurity content: Ammonia: ≤0.02%
Optical Rotation: +8.9 to +9.5°
CBNumber: CB1369616
Molecular Formula: C6H12ClN3O3
Molecular Weight: 209.63
MDL Number: MFCD00151027
MOL File: 5934-29-2.mol
Melting point: 254 °C (dec.)(lit.)
alpha: 9.9 º (c=11, 6 N HCl)
Density: 1.49 g/cm3
bulk density: 770kg/m3

vapor pressure: <1 hPa (20 °C)
storage temp.: Store below +30°C.
solubility: H2O: 100 mg/mL
form: Solid
color: White
Odor: odorless
PH Range: 3 - 4 at 105 g/l at 25 °C
PH: 3.5-4.5 (100g/l, H2O, 20℃)
biological source: non-animal source

optical activity: [α]20/D +9.5±0.5°, c = 5% in 5 M HCl
Water Solubility: 169.9 g/L (20 ºC)
Merck: 14,4720
BRN: 4168261
InChIKey: CMXXUDSWGMGYLZ-XRIGFGBMSA-N
LogP: -3.32 at 25℃
CAS DataBase Reference: 5934-29-2(CAS DataBase Reference)
EWG's Food Scores: 1
FDA UNII: X573657P6P
UNSPSC Code: 41116107
NACRES: NA.77

FIRST AID MEASURES of L-HISTIDINE MONOHYDROCHLORIDE MONOHYDRATE:
-Description of first-aid measures
*General advice:
Show this material safety data sheet to the doctor in attendance.
*If inhaled:
After inhalation: 
Fresh air.
*In case of skin contact: 
Take off immediately all contaminated clothing. 
Rinse skin with
water/ shower.
*In case of eye contact:
After eye contact: 
Rinse out with plenty of water. 
Call in ophthalmologist. 
Remove contact lenses.
*If swallowed:
After swallowing: 
Immediately make victim drink water (two glasses at most). 
Consult a physician.
-Indication of any immediate medical attention and special treatment needed.
No data available

ACCIDENTAL RELEASE MEASURES of L-HISTIDINE MONOHYDROCHLORIDE MONOHYDRATE:
-Environmental precautions:
Do not let product enter drains.
-Methods and materials for containment and cleaning up:
Cover drains. 
Collect, bind, and pump off spills. 
Observe possible material restrictions. 
Take up dry. 
Dispose of properly. 
Clean up affected area.

FIRE FIGHTING MEASURES of L-HISTIDINE MONOHYDROCHLORIDE MONOHYDRATE:
-Extinguishing media:
*Suitable extinguishing media:
Carbon dioxide (CO2) 
Foam 
Dry powder
*Unsuitable extinguishing media:
For this substance/mixture no limitations of extinguishing agents are given.
-Further information:
Prevent fire extinguishing water from contaminating surface water or the ground water system.

EXPOSURE CONTROLS/PERSONAL PROTECTION of L-HISTIDINE MONOHYDROCHLORIDE MONOHYDRATE:
-Control parameters:
--Ingredients with workplace control parameters:
-Exposure controls:
--Personal protective equipment:
*Eye/face protection:
Use equipment for eye protection. 
Safety glasses
*Body Protection:
protective clothing
*Respiratory protection:
Recommended Filter type: Filter A 
-Control of environmental exposure:
Do not let product enter drains.

HANDLING and STORAGE of L-HISTIDINE MONOHYDROCHLORIDE MONOHYDRATE:
-Conditions for safe storage, including any incompatibilities:
*Storage conditions:
Tightly closed. 
Dry.

STABILITY and REACTIVITY of L-HISTIDINE MONOHYDROCHLORIDE MONOHYDRATE:
-Chemical stability:
The product is chemically stable under standard ambient conditions (room temperature).
-Possibility of hazardous reactions:
No data available