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E 160B

CAS Number : 542-40-5
EC  Number : 208-810-8
Molecular Formula :     C24H28O4
Molecular Weight  :     380.5

E 160b  is the code given to bixin used as a coloring additive in the food industry. 
This code is based on the European Union’s food codes, which correspond to isolated natural colorings, such as Isobixin and Norbixin.

Besides, E 160b  is a coloring of plant origin that is extracted from Annatto Bixa Orellana tropical tree’s seed husks, which is mainly grown in countries of the American continent such as Costa Rica, Mexico (where it is called Acotillo), Ecuador, Colombia, Venezuela and in certain regions of Peru such as the Andean region.

While E 160b  refers to the raw extract, E 160b  is the fat-soluble coloring part, and E 160b  is the water-soluble part. 
All of them have coloring capacity, which since being extracted from plants acquires the name of natural coloring.
E 160b  can be extracted directly from Bixa Orellana seeds, either by washing them with oils or solvents such as chloroform and acetone, or by being added an aqueous solution of Potassium Hydroxide to produce E 160b , a water-soluble substance. 
E 160b  can be used in different industries.
E 160b  is used  in food products.

E 160b is a natural food colouring isolated from the seeds of the annatto tree(Bixa orellana). 
E 160b is the name of the crude extract, whereas bixin is the fat-soluble colour and norbixin the water-soluble colour.

E 160b  is used especially to give orange color to children. 
E 160b  is a mixture consisting of yield that has colorant equipment. 
Food dyes are also used in the food and pharmaceutical industries for overprotective masking in the final product. 
Food dyes are not used directly by the final home owners. 
During industrial food production, “g/kg” is used in addition to product descriptions.

Typical uses of E 160b :
E 160b  is used to give orange color in all kinds of sweets and confectionery manufacturing.

Physical Description of E 160b
E 160b extracts occur as dark red solutions, emulsions, or suspensions in water or oil or as dark red powders. 
The extract is prepared from annatto seeds, Bixa orellana L., using a food-grade extraction solvent. 
E 160b is the principal pigment of oil-soluble annatto extracts. 
E 160b is the principal pigment of alkaline water-soluble annatto extracts.

Common Uses of E 160b
E 160b extracts have been used for over two centuries as a food color, especially in cheese, and various types are now used in a wide range of food, drugs and cosmetic products, including butter, margarine, salad dressing, ice cream and other frozen desserts, confectionery, egg products, fish and fish products, meat products, cereals, cakes, beverages, soup mixes, fruit products, snacks, bakery products, spices and seasonings, and sausage casings.

E 160b is an orange coloring pigment with aesthetic enhancing activities. 
E 160b is soluble in oil and available in liquid form. 
Recommended for oil products and decorative cosmetics.

E 160b is a diterpenoid.
E 160b is under investigation in clinical trial NCT03820245 (Effect of Short-term Annatto Carotenoids Supplementation on Oxidative Stress Status in Healthy Individuals).

Applications of E 160b:  
Synthetic food colours are used to enhance the original colours associated with a given product. 
Also due to light, air, temperature and moisture many time product's colour fade or disappear, so colour are added to give a colourful identity to the colourless or dull looking foods. 
Also colours are used to provide a colourful appearance to "fun foods."

-Bakery Products
-Meat Products
-Soaps & Cosmetics
-Dairy Products
-Ice-Creams & Soft Drinks
-Salad dressing
-Instant food
-Cake mixes, etc.

Almost all confectionery has added colourants in them. 
Most common ones are candies, jellies, coated candies and chocolates.

-Jellied candy
-Hard candy
-Pan-coated candy
-Chewing gum
-Candy wafers

Cosmetic & Personal Care Products:

-Lotions Creams
-Eyeliners & Eye Shadows
-Liquid Soap
-Bath Soap
-Hair Oi
-After Shave
-Skin Creams

E 160b is a small tree of the Bixa genus. 
The plant became known for the red pigment found in the seeds and is widely used as a food coloring alternative to similar synthetic products. 
The dye is obtained by extracting it from seeds and used in powder form.

E 160b is a dark orange liquid, obtained from the Annatto seed .
E 160b is produced using a water-alkaline method of extraction followed by neutralization with inorganic acid and diluted in a basic solution. 
E 160b is soluble in water.
E 160b water soluble belongs to norbixin category which is in powder form. 

There are two types of E 160b water soluble products: 
E 160b is spray dried alkaline extract of annatto seeds. 
E 160b is completely soluble in water. 
E 160b has low tinctorial strength. 
E 160b can be used in food applications with pH close to neutral. 

E 160b is traditionally used to color dairy products like natural cheddar cheese, yogurt, dairy drinks, and ice cream where a water-soluble color works better. 
In cheese, E 160b binds to dairy proteins during cheese making, imparting excellent color and stability.
E 160b is the main pigment in annatto extract that is of interest to cheesemakers. 
E 160b is water soluble, meaning it will easily mix with water. 
In cheese making, E 160b extract is added to milk, which is predominantly water, so water solubility is important.

E 160b , a carotenoid, is extracted from the orange coloured outer coat surrounding the seeds of the shrub Bixa orellana. 
When E 160b  is initially extracted, it is known as Bixin. 
E 160b  is the Oil Soluble form of annatto, the primary ingredient surrounding the seeds in the fruit of the plant. 
E 160b  can be turned into Norbixin, which is truly water soluble–reducing the need for emulsifiers or gums. 
E 160b is a unique attribute of annatto within the carotenoid family.

E 160b, otherwise known as Norbixin, Orlean or Natural Orange (industrial reference number E 160b) is a yellow-reddish colourant with a carotene structure. 
E 160b is extracted from the Bixa Orellana plant which belongs to the Bixaceae family and originates in the Amazon Rainforest, thriving in India and Central America.
With oval red-veined leaves shaped somewhat like a heart, the Bixa Orellana shrub has clusters of pinkish-white flowers and fruits enclosed in fuzzy pods. 
E 160b is from the fruits of this plant that we extract the pigment so vital for the colourant.

Properties and benefits of E 160b
E 160b has been used since ancient times to colour foodstuffs and cosmetics. 
Native Indians from the Ecuador region would use annatto paste to dye their hair, whereas the indigenous peoples from Amazonia used it as an insect repellent as well as an antidote to poison mixed with tapioca.
In India E 160b is known as sindoor and thought to be auspicious for marriages. 
Indeed, E 160b is applied to the middle of the forehead to show that a woman is married.

Last but not least, the Aztecs used this natural colourant as war paint, but also for traditional rituals that marked birth and death within the community.
According to recent research, E 160b is also beneficial to our health, helping us to recover from diarrhoea, a high temperature, diabetes, water retention, a swollen stomach, hepatitis and malaria. 
E 160b is also used as an antioxidant and applied directly to burns, vaginal infections and insect bites.

Uses for E 160b
E 160b is used both in Europe and the United States as a colourant for cheeses and other foodstuffs. 
E 160b imparts several shades of yellow, but also orange and even red.
In Italy its use as a colourant for desserts, ice-cream, liqueur, bakery products, smoked fish, etc., is also permitted.
The cosmetics industry makes extensive use of it for make-up (it is the reason for the redness in lipstick), but it also turns up in creams, toothpaste and soap.

E 160b is an orange-red condiment and food coloring (E number 160b) derived from the seeds of the achiote tree (Bixa orellana). 
E 160b is often used to impart a yellow or orange color to foods, but sometimes also for its flavor and aroma. 
Its scent is described as "slightly peppery with a hint of nutmeg" and flavor as "slightly nutty, sweet and peppery".
E 160b and its extracts are now widely used in an artisanal or industrial scale as a coloring agent in many processed food products, such as cheeses, dairy spreads, butter and margarine, custards, cakes and other baked goods, potatoes, snack foods, breakfast cereals, smoked fish, sausages, and more.

Substance identity

EC / List no.: 208-810-8
CAS no.: 542-40-5
Mol. formula: C24H28O4

Hazard classification & labelling of E 160b 
According to the majority of notifications provided by companies to ECHA in CLP notifications no hazards have been classified.

E 160b  predicted as likely to meet criteria for category 1A or 1B carcinogenicity, mutagenicity, or reproductive toxicity, or with dispersive or diffuse use(s) where predicted likely to meet any classification criterion for health or environmental hazards, or where there is a nanoform soluble in biological and environmental media
E 160b  indicated, in 2009, as being intended to be registered by at least one company in the EEA.

E 160b  for which classification and labeling data have been submitted to ECHA in a registration under REACH or notified by manufacturers or importers under CLP. 
Such notifications are required for hazardous substances, as such or in mixtures, as well as for all substances subject to registration, regardless of their hazard.
E 160b  listed in the EINECS, ELINCS, or NLP inventories.

6,6'-diapo-ψ,ψ-carotenedioic acid
6,6'-diapo-ψ,ψ-carotenedioic acid
(2E,4E,6E,8E,10E,12E,14E,16E,18E)-4,8,13,17-tetramethylicosa-2,4,6,8,10,12,14,16,18-nonaenedioic acid
6,6'-diapo-.psi.,.psi.-carotenedioic acid
6,6'-Diapo-psi,psi-carotenedioic acid
Norbixin (cis/trans mixture)
(2E,4E,6E,8E,10E,12E,14E,16E,18E)-4,8,13,17-tetramethylicosa-2,4,6,8,10,12,14,16,18-nonaenedioic acid
E 160b
EINECS 208-810-8
2,4,6,8,10,12,14,16,18-Eicosanonaenedioic acid, 4,8,13,17-tetramethyl-
annatto seed extract (solvent-extracted norbixin)
bixa orellana seed extract (solvent-extracted norbixin)
annatto seed extract (alkali-processed norbixin, acid precipitated)
annatto seed extract (alkali-processed norbixin, not acid precipitated)
bixa orellana seed extract (alkali-processed norbixin, acid precipitated)
(ALL-E)-4,8,13,17-TETRAMETHYL-2,4,6,8,10,12,14,16,18-EICOSANONAENEDIOIC ACID
bixa orellana seed extract (alkali-processed norbixin, not acid precipitated)
(2E,4Z,6E,8E,10E,12E,14E,16E,18E)-4,8,13,17-Tetramethyl-2,4,6,8,1 0,12,14,16,18-icosanonaenedioic acid
(2E,4E,6E,8E,10E,12E,14E,16E,18E)-4,8,13,17-Tetramethyl-2,4,6,8,10,12,14,16,18-icosanonaendisäure [German] [ACD/IUPAC Name]
(2E,4E,6E,8E,10E,12E,14E,16E,18E)-4,8,13,17-Tetramethyl-2,4,6,8,10,12,14,16,18-icosanonaenedioic acid [ACD/IUPAC Name]
(2E,4E,6E,8E,10E,12E,14E,16E,18E)-4,8,13,17-tetramethylicosa-2,4,6,8,10,12,14,16,18-nonaenedioic acid
2,4,6,8,10,12,14,16,18-Eicosanonaenedioic acid, 4,8,13,17-tetramethyl-, (2E,4E,6E,8E,10E,12E,14E,16E,18E)- [ACD/Index Name]
208-810-8 [EINECS]
542-40-5 [RN]
6,6′-diapo-ψ,ψ-carotenedioic acid
6,6′-diapo-ψ,ψ-carotenedioic acid
6,6'-Diapo-ψ,ψ-carotenedioic acid
Acide (2E,4E,6E,8E,10E,12E,14E,16E,18E)-4,8,13,17-tétraméthyl-2,4,6,8,10,12,14,16,18-icosanonaènedioïque [French] [ACD/IUPAC Name]
all-trans norbixin
2,4,6,8,10,12,14,16,18-Eicosanonaenedioic acid, 4,8,13,17-tetramethyl- [ACD/Index Name]
33261-80-2 [RN]
33261-81-3 [RN]
4,8,13,17-tetramethylicosa-2,4,6,8,10,12,14,16,18-nonaenedioic acid
6,6'-Diapo-6,6'-carotenedioic acid
Annatto (E 160b)
annatto seed extract (alkali-processed norbixin, acid precipitated)
annatto seed extract (alkali-processed norbixin, not acid precipitated)
annatto seed extract (solvent-extracted norbixin)
Diapocarotene-6,6'-dioic acid
E 160b
E160 (Norbixin)
EINECS 208-810-8

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